犬急性水肿型胰腺炎胰液外引流模型的制备及胰腺外分泌功能的观察.docVIP

犬急性水肿型胰腺炎胰液外引流模型的制备及胰腺外分泌功能的观察 　　作者：金约朋1,程水兵2,周蒙滔1,张启瑜1　　　作者单位：(温州医学院第一附属医院，浙江 温州 325000，1.普外科;2.创伤外科) 　　【摘要】目的 建立犬急性水肿型胰腺炎胰液外引流模型，观察胰腺外分泌的功能。方法 健康杂种犬12只，分为对照组和胰腺炎组，每组6只。制备犬胰液外引流模型后24 h，5%牛磺胆酸钠(0.5 mL/kg)1 mL/min胰管逆行注射制备急性水肿型胰腺炎模型。每12 h收集胰液一次，测定胰液的分泌量，胰液中淀粉酶、脂肪酶、总蛋白、pH值和主要电解质含量。制模后在不同时段采血，检测血中的淀粉酶、脂肪酶的浓度。取胰腺组织行病理学检查。结果 成功建立犬急性水肿型胰腺炎胰液外引流模型。胰腺炎组胰液分泌量，胰液中淀粉酶、脂肪酶、总蛋白、HCO3-含量和pH值低于对照组(P0.05)，Cl-高于对照组(P0.05)。胰液中的Na+、K+、Ca2+、Mg2+胰腺炎组与对照组差异无统计学意义(P0.05)。结论 本模型适用于急性水肿型胰腺炎胰腺外分泌功能的研究。 　　【关键词】 胰腺外分泌;模型，动物;胰腺炎;犬 　　Abstract Objective To establish dogs pancreatic juice external drainage models of the edematous pancreatitis for the observation on pancreatic exocrine secretion function. Methods Twelve healthy dogs were randomly divided into control group and pancreatitis group， each group had six dogs. The dogs pancreatic juice external drainage models of the edematous pancreatitis was induced by injecting 0.5 mL/kg of 5% sodium taurocholate with the speed of 1 mL/min into the pancreas via pancreatic duct after 24 h of the preparation of dogs pancreatic juice external drainage models. Pancreatic juice flow and the output of amylase， lipase， protein， pH， HCO3-， Na+， K+， Cl-， Ca2+， Mg2+ in pancreatic juice were determined for every 12 h. Output of amylase and lipase in blood was determined in different times in pancreatitis group. Pancrease pathology of dog were observed under light microscope. Results The dogs pancreatic juice external drainage models of the edematous pancreatitis were established successfully. Compared with the control group， in pancreatitis group， pancreatic juice flow and output of amylase， lipase， protein， HCO3- in pancreatic juice decreased (P0.05)， output of Cl- in pancreatic juice increased (P0.05)， output of Na+， K+， Ca2+， Mg2+ in pancreatic juice had no difference between two groups (P0.05). Conclusion The dogs pancreatic juice external drainage model is suitable for the study of function of edematous pancreatic exocrine secretion. 　　Key wo