高纯度藻蓝蛋白分离纯化及光谱特性研究.pdfVIP

1999 ; 26 (5) 　　　　 生物化学与生物物理进展 　　 Prog. Biochem. Biophys. ·457 · human and rat CN TF . J Neurochem , 199 1 , 57 ( 3 ) : 1003 ～ of B as ic M edical S cience , A cademy of M il it ary 10 12 M edical S ciences , B eij i ng 100850 , China ) ; Q IU 5 　咸海青 , 范 　明, 甘思德 , 等 ( Xian H Q , Fan M , Gan S D , et al) . 人睫状神经营养因子的原核表达纯化及其生物效应. 中 ZongYin ( M edical Univers ity of Chong qi ng , 国应用生理学杂志 ( Chinese J Appli Phsiol) , 1996 , 12 ( 1) : Chong qi ng 400046 , China) . 2 1～24 Abstract 　 Renat uration of human cilinary 6 　Daniel M B , Stuart J E. Protein Met hods. New York : WileyLiss Press , 199 1. 50～160 neurot rop hic factor exp ressed in recombinant 7 　Mar ston F A O , Freedman F B . Recovery and reaction of recombi bacteria , by gel filit ration chromatograp h , was nant p roteins. Biochem Soc Trans , 1988 , 16 ( 1) : 10 1～103 st udied. It was showed t hat t he renat uration rate by 8 　Mar ston F A O , Angal S , Lowe P A , et al . Scaleup of t he t his way was higher t han t hat by dilution and dialy recovery and reaction of recombinant p roteins. Biochem Soc Trans , 1988 , 16 ( 1) : 112～115 sis , and t he p rotein was p urified at t he same time . It 9 　Doonan S , Walter J M . Downst ream p rocessing of p rotein was a simple , rapid , good rep roducibilit y and p roduct s. Biochem Soc Trans , 1990 , 18 ( 1) : 23 1～234 efficient p rocedure which can be used to p roduce