三株新城疫广西分离株全基因组序列的测定及分析.pdfVIP

第 21 卷 3 期 中 国 病 毒 学 21 （3 ）：261-266 2006 年 5 月 VIROLOGICA SINICA May 2006 三株新城疫广西分离株全基因组序列的测定与分析* 1** 1 2 1 1 谢芝勋 ，唐小飞 ，董建宝 ，刘加波 ，庞耀珊 ， 1 1 邓显文 ，谢志勤 （1. 广西兽医研究所，广西南宁，530001; 2. 广西大学动物科学技术学院，广西南宁，530005 ） Sequencing and Analysis of the Genomes of Three Newcastle Disease Viruses Isolated from Guangxi 1** 1 2 1 1 XIE Zhi-xun ，TANG Xiao-fei ，DONG Jian-bao ，LIU Jia-bo ，PANG Yao-shan ， 1 1 DENG Xian-wen ，XIE Zhi-qin (1.Guangxi Veterinary Research Institute, Nanning, 530001, China; 2. College of Animal Sciences and Technology, Guangxi University, Nanning, 530005, China) Abstract ：Eight pairs of specific primers were designed and synthesized based on the entire genomic sequence data of Newcastle Disease Virus (NDV) present in GenBank. Complete nucleotide sequences of GX7/02, GX9/03 and GX11/03 strains were acquired by reverse transcription-polymerase reaction. The entire genomic sequences of three NDV strains consisted of 15 192 nt, which was the same in length to 7 NDV ZJ1, U.S/Largo/71, Italy/2736/00 strains, but was 6 nt longer than LaSota, Clone-30 and B1 strains in GenBank. The additional 6 nt motif was located in the non-coding region