应用RACE法分离与克隆猪GPX2基因研究_英文_.pdfVIP

A gricu ltu ra l S c ience Te chno lo gy, 2008 , 9 ( 1) : 24 - 28 Cop yrigh t 2008 , In fo rm a tio n In stitu te o f HAA S. A ll righ ts re se rve d. A g ricu ltu ra l B io te chno lgy Study on the Clon ing and Isolation of su s scrofa GPX2 Gene by RACE M eth od 1, 2 1, 2 2 1, 2 2 ZHAO Hua , ZHOU J ichang ,L I Jungang , ZHAO Ying , WAN G Kangn ing 1. The Cen ter of F u tu re A g ricu ltu re f or H um an H ea lth, S ichuan A g ricu ltu re Un iversity, Ya ′an 625014; 2. A n im a l N u trition Institu te , S ichuan A g ricu ltura l Un i versity, Ya ′an 625014 Ab stra c t　[O bjective] U singmolecu lar b iotechnology to clone the sus scrof a GPX 2 gene. [M ethod] U sing total RNA of sus scrof a duodenum as temp late, degenerated p rim er p airs were designed according to the homology alignm ent analysis of GPX 2 gene of hum an, rat, mou se, dog and cattle. A sus scrof a GPX 2 gene sequence of 330 bp was obtained by RTPCR app lication m ethod. Prim eswere designed resp ectively according to the known sequence, sus scro f a GPX 2 gene was isolated and cloned by 3RACE and 5RACE m ethod and analyzed the gene sequence. [ Result] A mRNA sequence of 924 bp was suc cessfully cloned and isolated in th is research. This sequence contained comp lete 3 ’end and had higher sequence homology w ith hum an,mou se, cattle and dog GPX 2 gene, and there was codon called TGA which encoding Sec on the position of No. 114 - 116 gene. [ Conclusion ] Sequence alignm ent analysis ( ) showed that the cloned gene was sus scrof a GPX 2 gene NCB I GenB ank database, the