F—Z液与Belzer液、HC—A液在细胞水平的比较和低温保护剂作用机理的初步探索.pdfVIP

F--Z液与Belzer液、HC—A液在细胞水平的比较 和低温保护剂作用机理的初步探索 我们对现有的保存液Hc—A液进行改进，初步改进之结果命名为F—z液。 保存效果进行了比较，并得到以下四个结论：1．对Hc—A液作进一步的改良的 可能性是存在的；2．作为初步改良的结果，在减轻保存损伤和维持保存后的活力 两方面，F—z液都优于Hc—A液，达Belzer液相似的水平；3．保存所致损伤， 复温后才能充分表现出来：随着保存时间的延长，越接近能够承受的保存时间的 “临界点”，总体上所受损伤越重，复温后表达损伤的速度也越快；4．保存一复温 后的活力变化具波动性和周期性，在不同的周期内，存在作“最适保存一复温时 间 范围 ”。 在本论文的第二部分，试图对尚未完全明了的低温保护剂的作用机理作进 行初步的探索。方法是通过观察染料复温后在细胞内浓聚及扩散过程，推测降温 过程中细胞内冰晶形成过程。结论：低温保护剂的作用机理就在于降低冻存液的 冰点，使之等于或低于细胞内液，为降温过程的早期，细胞膜内侧完接的球形冰 层的形成创造条件。在此基础上，对该领域内几个机制相关问题进行了讨论。 关键词F—z液Belzer液HC—A液低温保护剂玻璃化 the effectofF—Z solution、HC—A Comparingpreservation solution andBelzer the of solutionexploringprinciplecryoprotectants to Wemadeaselection HC—A solutionthathas improve been widelyemployed in and tobesatisfiedrenal outcomeof proved preservation，the primary improvement WaS namedF—Zsolution．Aftertwo hard work．weachieved some years conclusion incellular to HC—Asolution level：1．Possibility is the improve being；2．As primary outcomeof solutionis than better HC—A improvement，F—Z solution．arrivesthe vitalforce in I-R and the solution injuryimproving ofBelzer level deereasing similar after Can only rewarming， present aRer completely injury preserve．rewarmimg；3．I-R it after I-R rewarming-4- thesustainableis，more presents moreserous injury quickly and fluctuation forceafter periodicity，during vital presents The preserve-rewarmimg bea‘‘best increasethe can time range ”to Deriodthere preserve-rewarmimg every after in vitalforce theory． preserve-rewarmimg ofthis the of that second cryopretectants Inthe exploredprinciple part paper，we thanhalf a after more centuryverysimple stillunclear widelyemployed by being of forwarda basethe outcome，we hypothesis： experiment’S put experiment．0n也e thesolution’S of isthat Can down The put cryopretectants cryopretectants principle it thatafull make evenunderintracellular to fluid’S，to possible freezingpoint，equal to atthe of membranecomes roundice inside cell being beginning layer preservated several ofthe ans