Repetitive exposure to a 60-Hz time-varying magnetic field induces DNA double-strand breaks and apoptosis in human cells》.pdf

Biochemical and Biophysical Research Communications 400 (2010) 739–744 Contents lists available at ScienceDirect Biochemical and Biophysical Research Communications journal homepage: www.else /locate/ybbrc Repetitive exposure to a 60-Hz time-varying magnetic field induces DNA double-strand breaks and apoptosis in human cells Jiyeon Kim a, Chang Seung Ha b, Hae June Lee b, Kiwon Song a,⇑ a Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 120-749, Republic of Korea b School of Electrical Engineering, College of Engineering, Pusan National University, Busan 609-735, Republic of Korea a r t i c l e i n f o a b s t r a c t Article history: We investigated the effects of extremely low frequency time-varying magnetic fields (MFs) on human Received 26 August 2010 normal and cancer cells. Whereas a single exposure to a 60-Hz time-varying MF of 6 mT for 30 min Available online 15 September 2010 showed no effect, repetitive exposure decreased cell viability. This decrease was accompanied by phos- phorylation of c-H2AX, a common DNA double-strand break (DSB) marker, and checkpoint kinase 2 Keywords: (Chk2), which is critical to the DNA damage checkpoint pathway. In addition, repetitive exposure to a Time-varying MF time-varying MF of 6 mT for 30 min every 24 h for 3 days led to p38 activation and induction of apoptosis DNA double-strand break (DSB) in cancer and normal cells. Therefore,