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《Molecular identification of species in JuglandaceaeA tiered method》.pdf
Journal andEvolution doi：10．111 1．00116．x
ofSystematies 49(3)：252-260(2011) 1／j．1759-6831．201
ResearchArticle
Molecularidentificationof in
speciesJuglandaceae：
Atieredmethod
1 1
1，一Xiao．GuoXIANG ZHANG LU LI堆
1，2Jing．Bo An—MingRui．Qi
100093，China)
2(GraduateUniversityofChineseAcademyofSciences,Beijing100049，China)
AbstractDNA identification data．A
isamethodof and DNA tiered
barcoding species recognitionusing sequence
method for 1
ormultilocushasbeenrecommended this 96individuals
barcodingplantspecies．Instudy,wesampled
9 and54 of to the ofthefour loci
utility
representinggenera speciesJuglandaceaeinvestigate potentialbarcoding
intemaltranscribed resultsshowthatallfourDNA are
(rbcL，matK，trnH-psbA，andspacer(ITS))．Our regions
to and thefourtestedDNA hasthemostvariable
easyamplifysequence．In regions．ITS
hasthe canbe identifiedmatK．At
least．At level．sevenofnine level．ITS
generic genera efficiently by species
has thanthe to inthewhole showed
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