《Testing four proposed barcoding markers for the identification of species within Ligustrum L.(Oleaceae)》.pdfVIP
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《Testing four proposed barcoding markers for the identification of species within Ligustrum L.(Oleaceae)》.pdf
Evolution
Journal and
ofSystematics 49(3):213-224(2011)
Article
Research
four markersfortheidentificationof
proposedbarcoding
Testing
withinIlln LUleaceae
selcespecies)eaecaeLip,ustrumLtgustrumlo(.L .I
GU2Jun—XiaSU2Ruo·ZhuLIN LI 1Pei-GenXIAO+
1,2Jing 2Rui—Qi
100193,Chim)
100093,China)
AbstractDNA isabiological thatUSeSshortandstandardizedorDNA to
barcoding technique genes regions
facilitate identification.DNAhasbeenused inseveralanimaland
species barcoding successfully plantgroups.
occur theworldandareusedasmedicinalin
China.Therefore,
Ligustrumroleaceae)specieswidelythroughout plants
of inthis is DNA thenuclear
theaccurateidentification
species genusnecessary.Fly
ribosomalinternaltranscribed three regions(rbcL,matic:,andtrnH-psbA),
spacer(ITS)andchloroplast(cp)DNA
wereusedtodifferentiatewithin methodsand
species Ligustrum.BLAST.character-basedmethod,tree—based
wereusedto themolecularidentificationofthechosenmarkersfor
TAXONDNA
analysis investigate capabilities
92 20 ofthis resultsshowedthattheITS have
discriminatingsamplesrepresentingspecies genus.The sequences
themostvariableinformation,followed rb吐.All ofthefour
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