《基于“酥梨”芽转录组的简单序列重复（SSR）标记开发 Development of genic SSR markers from transcriptome sequencing of pear buds》.pdfVIP

Yue et al. / J Zhejiang Univ-Sci B (Biomed Biotechnol) 2014 15(4):303-312 303 Journal of Zhejiang University-SCIENCE B (Biomedicine Biotechnology) ISSN 1673-1581 (Print); ISSN 1862-1783 (Online) /jzus; E-mail: jzus@ Development of genic SSR markers from *# transcriptome sequencing of pear buds 1 1,2 1 †‡1 †‡1 Xiao-yan YUE , Guo-qin LIU , Yu ZONG , Yuan-wen TENG , Dan-ying CAI 1 ( State Agricultural Ministry Key Laboratory of Horticultural Plant Growth, Development Quality Improvement, Department of Horticulture, Zhejiang University, Hangzhou 310058, China) (2 College of Agriculture, Guizhou University, Guiyang 550025, China) †E-mail: ywteng@; dycai@ Received Sept. 6, 2013; Revision accepted Dec. 3, 2013; Crosschecked Mar. 13, 2014 Abstract: A total of 8375 genic simple sequence repeat (SSR) loci were discovered from a unigene set assembled from 116 282 transcriptomic unigenes in this study. Dinucleotide repeat motifs were the most common with a frequency of 65.11%, followed by trinucleotide (32.81%). A total of 4100 primer pairs were designed from the SSR loci. Of these, 343 primer pairs (repeat length ≥15 bp) were synthesized with an M13 tail and tested for stable amplification and polymorphism in four Pyrus accessions. After the preliminary test, 104 polymorphic genic SSR markers were devel- oped; dinucleotide and trinucleotide rep