分解脂肪的微生物分析方法.DOCVIP

Lipolytic micro organisms enumeration Reason of change: Distribution list: Division Director CLF X QB X Microbiology Division X Nutrients Division Residues Division 1 Scope and field of application适用范围和领域 The method for the detection and enumeration of lipolytic micro organisms in foodstuffs and other materials. 本方法适用于食品和其他材料中分解脂肪微生物的检测和步骤。 2 Principle of the method方法的原理 This method is based on Anderson (1939).这一方法建立在Anderson (1939)的基础上。 The culture medium contains tributyrin as a reactant; degradation of this compound gives rise to clear zones surrounding the lipolytic colonies in the otherwise turbid culture medium. 培养基包含三丁酸甘油酯作为反应物，在浑浊的培养基中因分解这种混合物而清晰出现分解脂肪生物体菌圈。 3 Definitions and abbreviations定义和缩写 cfu colony forming units菌落单位 4 Reagents, materials, media试剂、原料、指示剂 Medium/reagen Abbreviation Advised maximal storage time1 方法/试剂 缩写 最长建议保存时间 Tributyrin Agar*), Ba TBA 1 month 4-10°C 三丁酸甘油酯 TBA 4-10°C 1个月 Glycerol tributyrate (Tributyrin)**) see manufacturers description 甘油三丁酸甘油酯 视生产商说明 1 The shelve life of the prepared media/solutions is given when stored in the dark. Media which can be kept longer than 1 month have to be stored in well closed (screw capped) bottles. *…….e.g. Tributyrin Agar [Merck Darmstadt cat. No. 1.01957.0500] **.......e.g. Glycerol tributyrate (Tributyrin) [Merck Darmstadt cat. No 1.01958.0100] 保存在黑暗处的制备好的溶液、指示剂的货架期：用瓶子密封贮存的指示剂能保存1个月以上 Use only media with a good quality, proven by suppliers quality certificate and/or ecometrical test results.使用合适指示剂的优良质量，通过供应商质量证书或检测结果予以证明。 Follow the manufacturer’s description on:制造商的描述为以下方面 Storage of the (powdered) media and reagents（粉末）指示剂和试剂的贮藏 Preparation of the media指示剂的制备 Storage of the prepared media制备好的指示剂的贮藏 5 Equipment仪器 Usual microbiological equipment.通常微生物技术仪器 6 Proc