第六章分子精品.pptVIP

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第六章分子精品.ppt

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第六章分子精品.ppt

The structure of a monomer of Lac repressor identifies several independent domains. Photograph kindly provided by Mitchell Lewis. Repressor protein binds to the operator and is released by inducer The crystal structure of the core region of Lac repressor identifies the interactions between monomers in the tetramer. Each monomer is identified by a different color. Photographs kindly provided by Alan Friedman. The crystal structure of the core region of Lac repressor identifies the interactions between monomers in the tetramer. Each monomer is identified by a different color. Photographs kindly provided by Alan Friedman. The crystal structure of the core region of Lac repressor identifies the interactions between monomers in the tetramer. Each monomer is identified by a different color. Photographs kindly provided by Alan Friedman. The crystal structure of the core region of Lac repressor identifies the interactions between monomers in the tetramer. Each monomer is identified by a different color. Photographs kindly provided by Alan Friedman. 操纵基因的鉴定小结 1. The lac genes are controlled by a repressor 2. The lac operon can be induced 3. Repressor is controlled by a small molecule inducer 4. Repressor protein binds to the operator 5. Binding of inducer releases repressor from the operator 6. Repressor is a tetramer 7. Repressor binds to three operators and interacts with RNA polymerase 8. Repressor is always bound to DNA 9. CAP functions in different ways in different target operons 10. CAP bends DNA Organization of Lac Operon and LacI Operon promoter Ribosome initiation The Lac Operon:When Glucose Is Present But Not Lactose Repressor Promoter LacY LacA LacZ Operator CAP Binding RNA Pol. Repressor Repressor Repressor mRNA Hey man, I’m constitutive Come on, let me through No way Jose! CAP The Lac O