苏丹草、甜高粱高粱不育系杂交试验及issr分子标记研究.pdfVIP

摘 要 本试验以苏丹草、甜高粱与高粱不育系为亲本进行杂交试验，旨在组配并选育出 产草量高、结实率高兼备适中含糖量的优良新品种。2011 年对所有供试父本进行系 统筛选，在2012 年的杂交选育过程中，对杂种F 1 进行农艺性状考察、杂种优势分析、 配合力分析和ISSR 分子标记分析。试验结果如下： （1）通过生育期选择、田间农艺性状和年产草量的分析与比较，我们对15 个供 试父本进行淘汰与选择。最终选定六个父本品种进行杂交试验，这六个入选父本为 DxK、T03、N0601 、N0605 、B95 和W010 。 （2 ）杂种优势分析和配合力分析结果基本一致。数据分析结合田间观察可知： 三个雄性不育系系列杂交种的综合表现比较结果为Tx622 系列杂交种HZ180 系列杂 交种Tx623 系列杂交种。 （3 ）对所得17 个杂交组合进行杂种优势分析和配合力分析，选出的优势杂交组 合为Tx622 ×DxK、Tx622 ×W010 和HZ180 ×N0601 三个杂交种。 （4 ）利用筛选出的5 条ISSR 引物对23 个供试材料进行ISSR-PCR 扩增反应共 得到44 条谱带，DNA 片段长度介于 100～2000bp ，其中32 条具有多态性，条带多 态率为 72.73%，这表明我们进行本次试验所用父母本和所得杂交组合多态性丰富， 杂种后代中出现了丰富的新性状类型。 关键词：苏丹草；甜高粱；高粱雄性不育系；杂种优势 The Research of Sudan Grass,Sweet Sorghum and The Grain Sorghum Sterile Lines Hybridization Properties and ISSR Abstract In this study,Sudan grass,sweet sorghum and the grain sorghum sterile lines were used for hybridization,to hybrid new varieties,possessing high grass yield,high solid rate and average stalk Brix.In 2011,all the tested male parents were systematacially investigated and some of them were selected for the following hybridization experiments in 2012.Some characteristics of F1 generation varieties were evaluated,such as agronomic traits, Heterosis,parents combing ability and ISSR molecular marker on the first -filial generation. The results were as follows: (1) According to procreating time,agronomic characteristics and annual fresh grass yield,six kinds of male parents were chosen for the hibridization experiments,which are DxK﹑T03﹑N0601 ﹑N0605 ﹑B95 and W010. (2) The Heterosis of F1 generation varieties was basically in agreement with the combining abilities of their parents.The comprehensive performance of the three mal