原创力文档a reusable optical biosensor for the ultrasensitive and selective detection of unamplified human genomic dna with gold nanostars：对于超灵敏和选择性扩增人基因组dna与金纳米检测的可重复使用的光学生物传感器.pdfVIP
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a reusable optical biosensor for the ultrasensitive and selective detection of unamplified human genomic dna with gold nanostars：对于超灵敏和选择性扩增人基因组dna与金纳米检测的可重复使用的光学生物传感器
Biosensors and Bioelectronics 74 (2015) 981–988
Contents lists available at ScienceDirect
Biosensors and Bioelectronics
journal homepage: /locate/bios
A reusable optical biosensor for the ultrasensitive and selective
detection of unamplified human genomic DNA with gold nanostars
Stefano Mariani a, Simona Scarano a, Jolanda Spadavecchia b,c, Maria Minunni a,n
a Dipartimento di Chimica “Ugo Schiff”, Università di Firenze, Via della Lastruccia 3-13, 50019 Sesto Fiorentino (FI), Italy
b Laboratoire de Reactivite de Surfaces, UMR CNRS 7197, Universite Pierre Marie Curie Paris VI, Site d ’Ivry Le Raphael, 94200 Ivry-sur-Seine, Francé ́ ́ ̈
c Laboratoire CSPBAT UMR7244, UFR Santé, Médecine et Biologie Humaine, Université Paris 13, 74 rue Marcel Cachin, 93017 Bobigny, France
a r t i c l e i n f o a b s t r a c t
Article history: A Surface Plasmon Resonance imaging (SPRi) based DNA sensors for the selective and ultrasensitive
Received 17 April 2015 human genomic DNA detection, directly extracted from lymphocytes (bypassing PCR amplification), is
Received in revised form reported. To achieve DNA detection, a rationally chosen star-shaped nanoparticle (NP), namely gold
29 June 2015 nanostar (AuNS), has been applied, for the first time, in a sandwich-like assay based on the selective
Accepted 30 July 2015
capturing of specific DNA targets and the subsequent signal amplification by a secondary DNA probe
Available online 1 Au
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