fish技术检宫颈癌组织中hterc、her-2基因表达及其临床意义.pdf

of andclinical hTERCan Expression significance cervicall scrapingsamples Abstract clinical ofhTERCandHER一2 To the andtheir significance Objecfiveexploreexpression andtoevaluatetheeffectandclinical ofHER一2andhTERCincervical gene significance Iesion． MethodsThefluorescentinsitu usedtodetectthe ofthe expression hybndization(FISH)was hTERCandHER··2 incervical andtheParaffin-·embeddedtissue samples gene scraping 45in sectionswithfiveProductionMethods．Thecervical weredividedinto scrapingsamples inClN in and50incervicalcancer．ThePara怖n-embeddedtissue normal．29I，38CINII．ⅡI dividedinto13innormal，12inCINI，20in ClNII．ⅡIand18incervicaI sectionswere CanCer． ResultsWedetected225 12casesofdirectsmear specimens，whichincluding method，60 casesofsaline casesoflowosmotic casesofTCT method method，20 method，70 production and63casesof tissuesectionsmethod．Thetotal success paraffin·embedded hybridization HER一2 for rateis76．4％：Theachievementratioofthe ofhTERCand the hybridization gene directsmear saline TCT IOWosmoticmethod method，themethod，theproductionmethod，the andthePa阳ffjn-embeddedtissuesectionmethodwere