mirna-34a在乳腺癌侵袭转移机制中的作用.pdf

·中文论著摘要· miRNA．374a在乳腺癌侵袭转移机制中的作用 目 的 方法 本实验标本共48例，分为高侵袭性组16例，低侵袭性组16例，正常腺体组 16例。每组中随机选取6例标本混合后分别进行高通量基因组学分析。通过生物 Western 检验用A_NOVA，两组间比较用SNK检验，相关性分析用Pearson分析。 结 果 value=-0．845)。 之间存在显著的负相关(pearson ．‘ ：口结论-，己 负调控作用。 关键词 乳腺癌；转移；基质金属蛋白酶14(MMP．14)；miRNA．374a ·英文论著摘要· Theroleofm删A．374aintheinvasiveand metastasis mechanismofbreastcancer ective Obj This wasconductedtoresearchtheroleofmiRNA．374aintheinvasion study and metastasisofbreast thetranslationofMMP-14 process cancer,throughregulating protein Methods 48human breasttumortissueswere 16 lligll obtained，includingpatients、析tll invasion the andmetastatic 6 low enteringhi曲invasive group(HS)，1patients晰tll invasion thelowinvasiveandmetastatic 16 entering group(LB)and patients埘th tumorstoachievenormalbreasttissuesasthecontrol of16 benign group．Each group were dividedintotwo first ofsixcaseswasusedfor specimensrandomly parts：thepart secondofthe10caseswasusedforverification MicroRNA-Arrayscreenings，thepart Realtime and were experimentby RT-qPCR Western