不同疾病患者血浆内毒素含量的测定及结果分析.docVIP

不同疾病血内毒素测定血浆内毒素临床意义l，51例肝硬化病人内毒素阳性率为49.02％，平均内毒素含量为74.97 pg/ml。31例发热、28例白血病、16例肺炎、12例肺癌、9例肝炎和14例肝癌患者组血浆内毒素阳性率分别为67.74％、67.86％、68.75％、100％、33.33％和57.14％，内毒素平均值分别为73.47、29.47、239.45、259.42、8.73和17.34 pg/ml，与正常对照组相比均有显著性差异。不同肝病之间及肺炎和肺癌之间均无显著性差异。结论 检测血浆内毒素含量有助于早期判断感染类型及是否存在内毒素血症，有利于临床及早诊治疾病。 关键词：疾病；内毒素；内毒素血症；检测方法 中图分类号：378.2 文献标识码：A Clinical significance of plasma endotoxin detecting ZHANG Jian-fang, XU Xiu-li,FAN Xin,SUN Yi-qun (Clinical Lab. of Xijing Hospital,the Fourth Military Medical University,Xi’an 710032,China) Abstract: OBJECTIVE To study the Clinical significance of plasma endotoxin detecting. METHODS Plasma endotoxin of patient and healthy people were detected by turbidimetric assay with limulus reagents. RESULTS Mean plasma entoxin of 30 healthy people was 3.30?.73pg/ml. Mean plasma entoxin of 51 hepatocirrhosis patients is 74.97 pg/ml and its positive rate is 49.02%. Mean plasma entoxin of other groups including fever，leucocythemia，pneumonia，lung cancer，hepatitis，liver cancer is 73.47、29.47、239.45、259.42、8.73 and 17.34 pg/ml, respectively.The positive rates of different groups above-mentioned are 67.74％、67.86％、68.75％、100％、33.33％ and 57.14％，respectively.All patients groups have notable difference to healthy people group, but there are no notable difference among different liver disease and lung disease. CONCULION Plasma endotoxin detecting is helping to estimate infection types and whether endotoxemia exists. It is also help doctors to make a diagnosis and give treatment on time. Key words: Endotoxin； Endotoxemia；Detecting method 收稿日期： 修回日期： 自1968年Levin和Bang发现并建立鲎试验方法以来，逐渐得到临床和药检部门的重视。尤其是在临床检验方面的应用更是飞速发展。随着鲎试验方法研究和测定仪器的不断完善，内毒素测定已由最初的定性凝胶法向灵敏度更高的微量定量方面发展。 据国外文献报道：正常人血浆中的内毒素水平一般在3pg/ml左右，然而由于前处理方法及测定原理的不同其值也存在着一定差异。随着血液前处理方法的标准化和国内血液前处理液的生产，我国临床内毒素定量动态检测水平提高，内毒素定量检测的标准化进程将会加速。鲎验检测内毒素，℃冰箱中保存1个月。取上述富血小板血浆0.05ml，加入装有0.45ml样品处理液中，混匀后在T01智能恒温仪上70℃保温10min，取出后立刻放入冰水浴中，即为待测血浆样品。 1.3.2测定分析 取待测血浆0.2ml直接加入反应主剂中，溶解后使用微量加样器转移至10×75mm标准玻璃反应管中（不