纤维堆囊菌SoF5-76产埃博霉素B分批发酵动力学的研究.pdfVIP

生物技术通报 ·研究报告· BIOTECHNOLOGY BULLETIN ( ) 2015, 31 2 :202-207 纤维堆囊菌 SoF5-76 产埃博霉素 B 分批发酵动力学研究 马利云  龚国利  王娜 （陕西科技大学生命科学与工程学院，西安 710021） 摘 要 ： 埃博霉素发酵生产体系复杂，且生产菌株较难操作，导致发酵参数测定困难，迄今为止未见关于埃博霉素发酵动 力学方面的报道。利用5 L 发酵罐，对纤维堆囊菌 （ ）产埃博霉素B（Epothilone B）的分批发酵动力学进行了 Sorangium cellulosum 研究，整个发酵过程中维持温度30℃、pH7.4。发酵结束时，菌体干重达3.00 g/L，埃博霉素B 产量达18.20 mg/L，葡萄糖含量为0.049 g/L。基于Logistic 方程和Luedking-Piret 方程，利用MATLAB 软件对其进行非线性拟合，构建了菌体生长、埃博霉素B 合成和葡萄 糖消耗的动力学模型。结果表明，该组模型能较好的拟合发酵过程。 关键词 ： 纤维堆囊菌；埃博霉素B ；分批发酵 ；动力学模型 ；MATLAB ；非线性拟合 DOI ：10.13560/ki.biotech.bull.1985.2015.02.030 Kinetics of Batch Fermentation of Epothilone B by Sorangium - cellulosum SoF5 76 Ma Liyun Gong Guoli Wang Na （ ， ，Xi ’an 710021 ） College of Life Science and Engineering Shaanxi University of Science Technology Abstract ： The fermentation system produced epothilone is complex, and the production strain is more difficult to operate, leading to difficulties in fermentation parameters measured, so far there is no reporting on Epothilone fermentation kinetics. In this paper, the batch fermentation kinetics of the epothilone B produced by Sorangium cellulosum SoF5-76 was studied in a 5 L fermentor. During the process, the temperature and pH were almost constant, 30℃, 7.4. When the fermentation finished, the dry cell weight, epothilone B production and residual glycerol could reach 3.00 g/L, 18.20 mg/L and 0.049 g/L. On the bases of Log