噻虫胺残留时间分辨荧光免疫分析与研究.docVIP

噻虫胺残留时间分辨荧光免疫分析研究 李明，盛恩泽袁玉龙刘晓凤华修德* （南京农业大学植物保护学院农药科学系，江苏 南京210095） 摘要：铕（Eu3+作为荧光标记物羊抗兔残留的时间分辨荧光免疫分析法TRFIA）。在最佳优化条件下，建立了噻虫胺的TRFIA标准曲线，抑制中浓度IC50）为2.07μg/L，最低检测浓度LOD，IC10）为0.0220 μg/LTRFIA除了对呋虫胺有较大交叉反应外（9.7%），与噻虫胺结构类似化合物没有明显的交叉反应。水、甘蓝和大米中的平均添加回收率为74.1-115.9%，相对标准偏差为3.7-11.7%。真实样品的检测结果表明TRFIA与气相色谱法具有高度的相关性（R2 = 0.9902）。研究说明建立的TRFIA能够高灵敏地检测农产品中噻虫胺的残留。 关键词：噻虫胺；；；时间分辨荧光免疫分析法 ay for quantitative determination clothianidin LI Ming, SHENG Enze, YUAN long, LIU Xiaofeng, SHI Haiyan, HUA Xiude, WANG Minghua* (Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China) Abstract: Europium (Eu3+)-labeled antibody was used as a fluorescent label to develop a highly sensitive indirect competitive time-resolved fluoroimmunoassay (TRFIA) for determination of clothianidin residues based on polyclonal antibody in agricultural samples. Under optimal conditions, the half-maximal inhibition concentration (IC50) and the limit of detection (LOD, IC10) of clothianidin were 2.07 and 0.0220 礸/L for the TRFIA,n respectively. The TRFIA was no obvious cross-reactivities with the analogues of clothianidin except for dinotefuran (9.7%). Analysis of clothianidin-fortified samples (water, cabbage and rice) by the TRFIA showed average recoveries from 74.1-115.9% with relative standard deviations of 3.7-11.7%. The results of TRFIA for the authentic samples were largely consistent with gas chromatography (R2 = 0.9902). The optimised TRFIA might become a sensitive and satisfactory analytical method for the quantitative monitoring of clothianidin residues in agricultural produces. Keywords: clothianidin; europium; plyclonal antibody; time-resolved fluoroimmunoassay 噻虫胺clothianidin）是噻唑环的新型烟碱类杀虫剂，以其新颖的作用机制、广谱高效的杀虫活性、灵活的使用方法，被广泛应用于防治危害水稻、蔬菜、果园、茶叶等作物的半翅目、鞘翅目、双翅目和某些鳞翅目害虫。随着人们对噻虫胺在农产品和环境中残留检测方法的研究也广泛开展。我国尚未制订噻虫胺残留的分析方法，为标准的制订提供依据。噻虫胺残留高效液相色谱法(HPLC) 和* 通讯作者(Author for correspondence)：王鸣华(1961-)，男，山西垣曲人，博士，教授，主要从事农药残留与环境毒理研究，电话：025E-mail: wangmha@