紫外应用简介.ppt

Ultraviolet-Visible（UV-Vis）Spectroscopy/紫外-可见光谱 Zhang Ying 51140606197 Ultraviolet-Visible（UV-Vis）Spectroscopy/紫外-可见光谱 Contents PART ONE Aims 1、Describe briefly how the absorbance of a UV-absorbing compound is obtained. 2、Explain the relationship between the absorption wavelength and the energy difference between the energy levels involved. 3、Calculate the molar absorptivity of a molecule from its absorbance, concentration and the path length. Aims 4、Recognize chromophores, and explain how conjugation and aromatic substituents can contribute to chromophores. 5、Explain how PH can affect the chomophore and the UV-Vis spectra of acidic and basic compounds. 6、Predict the absorbance maximum for unsaturated compounds using the woodward-Fieser rules. 7、Summarize some practical applications. Contents PART TWO: 2.1 Instrumentation 2.2 Selection Rules 2.3 Beer--Lambert Law 2.4 Chromophores 2.4.1 Role of the Solvent Contents PART TWO: 2.4.2 Effect of pH 2.4.3 Woodward--Fieser Rules 2.5 Application of UV Spectroscopy 2.5.1 UV in the Analysis of Pharmaceutical Preparations(药物制剂）. 2.5.2 UV Detection in Chromatography(色谱学). Instrumentation（仪器设备） There are several different types of UV-Vis spectrometers(分光光度计), with the usual instrument met in an laboratory being a double beam spectrophotometer（双光束分光光度计）, Which consists of a UV-visible light source（光源）, two cells through which the light passes, and a detector to measure the amount of light passing through the cells（吸收池）. There are basic spectrometers that measure the absorbance at a specific wavelength(特定波长), set by the user, and others that can scan the entire UV-Vis range. Instrumentation(仪器设备) Newer Spectrometers (分光光度计) are usually computer controlled