东南大学电子学院《现代光学基础》补充内容3——显微镜资料.pptVIP

第七章 共焦显微光学Chapter 7 Confocal Microscopy Optics;OUTLINE;7.1 Optical Microscopy;Microscopy: History;Microscopy: History;Microscopy: History;Microscopy: Importance;Simple Magnification;Image formation on Retina;Combination of Lens and Eye;Combination of Lens and Eye;Compound Microscope;Compound Microscope;Airy disks and resolution. (a-c) Airy disk size and related intensity profile (point spread function) as related to objective numerical aperture, which decreases from (a) to (c) as numerical aperture increases. (e) Two Airy disks so close together that their central spots overlap. (d) Airy disk