第四章超数排卵.pptVIP

动物繁殖学双语教学English-Chinish of Reproductiong in Farm Animal Training　manuai for embryo transfer in cattle 动物繁殖学双语教学 Superovulation Chapter 4 SUPEROVULATORY TREATMENTS Superovulation is a very inefficient低效率 method of obtaining oocytes from bovine ovaries and is likely to be replaced by other approaches within the next decade. 第四章 超数排卵 超数排卵处理 超数排卵是一种从母牛卵巢中获得卵子的低效率inefficient的方法，在下一世纪内这一方法将可能被替代。 Inefficient 低效率 动物繁殖学双语教学 However, superovulation results in about ten times more embryos than single ovum recovery. 但是，超数排卵处理的结果比单一的排卵得到的胚胎要多10倍。 动物繁殖学双语教学 Without superovulation, a usable embryo can be recovered about 60 percent of the time from normal donors by skilled technicians. 采用熟练的技术，正常的供体超排后可得到大约60%的可用胚。 动物繁殖学双语教学 Under similar conditions, superovulation usually yields an average of six usable embryos, although the variation is astounding令人惊骇的(Figure 1). 在相同的条件下，超数排卵平均可得到6枚可用胚，虽然有较大的差异astounding （表1）。 astounding令人惊骇的 动物繁殖学双语教学 Normally, no embryos are recovered from 20-30 percent of superovulated donors and only one to three embryos are obtained from another 20-30 percent (see Table 11). 正常情况下，20-30%超排的供体检不到胚胎，仅有2-0-30%的可以检到1-3枚胚胎（见表11） 动物繁殖学双语教学 An ideal response of five to 12 embryos is obtained from about one-third of the donors. However, a small percentage of donors yield more than 20 good embryos and, very rarely, more than 50. 大约1/3的供体可以获得5-12枚胚胎。但是，小部分供体可获得20枚好胚胎，极少数超过50枚。 FIGURE 1 Superovulated bovine ovary photographed during a surgical recovery procedure, Also note opening to fimbria伞, 动物繁殖学双语教学 The two generally accepted methods of superovulating cattle are based on two different gonadotrophins（促性腺激素）, although there are many minor（较少）variations of these methods. 2种基本的方法 牛的超排方法有2种不同促性腺激素 gonadotrophins方法，其他方法与这些方法的有一定的差异 gonadotrophins促性腺激素 minor 较少 动物繁殖学双语教学 The simplest is to give an intramuscular (i.m.) injection of 1 800-3 000 IU (usually 2 000-2 500 IU) of pregnant mares serum gonadotmphin (PMSG), more correctl