miR―140与膝关节骨性关节炎严重程度的相关性.docVIP

miR―140与膝关节骨性关节炎严重程度的相关性 　　【摘要】 目的：探析miR-140与膝关节骨性关节炎严重程度的相关性。方法：选择本科收治的膝关节骨性关节炎患者60例为骨性关节炎组（OA组），按LawrenceX线诊断标准，Ⅰ～Ⅳ级患者各15例。同时选取同期非骨性关节炎患者30例为对照组（主要为外伤患者）。应用实时荧光定量（PCR）检测上述两组患者膝关节液中miR-140的含量。比较两组及不同等级患者的miR-140表达水平。结果：OA组miR-140表达水平显著低于对照组，比较差异有统计学意义（P0.01）；miR-140表达水平随OA严重程度增加而降低，比较差异有统计学意义（P0.01）。结论：miR-140表达水平与膝关节骨性关节炎的严重程度呈负相关。 　　【关键词】 临床观察； 膝关节骨性关节炎； miR-140 　　【Abstract】 Objective：To study the correlation of miR-140 with the severity of knee osteoarthritis.Method：60 patients with knee osteoarthritis were selected as the OA group.According to Lawrence X-ray diagnosis standard，there were 15 cases from level Ⅰ to level Ⅳ respectively.30 cases without OA were selected as the control group at the same time（they were mainly trauma patients）.Through real-time fluorescence quantification，the content of miR-140 in the two groups was detected.The expression levels of miR-140 in the two groups and patients of different degrees were compared.Result：The expression level of miR-140 in the OA group was significantly lower than that in the control group，the difference was statistically significant（P0.01）.The expression level of miR-140 was reduced according with the increasing of the severity of knee osteoarthritis，the difference was statistically significant（P0.01）.Conclusion：The expression level of miR-140 is negatively correlated with the severity of knee osteoarthritis.. 　　【Key words】 Clinical observation； Knee osteoarthritis； miR-140 　　First-author’s address：The People’s Hospital of Xuwen County，Xuwen 524100，China 　　doi：10.3969/j.issn.1674-4985.2016.07.001 　　骨性关节炎（OA）是一种慢性关节病，典型特征为骨质增生，关节软骨破坏、变性等，多见于老年患者[1]。随着我国人口老龄化的加剧，OA的发病率出现逐年上升的趋势。该病发病率随年龄的增长而增加，特别是55岁之后OA发病率明显增加。OA的发病确切机理目前仍不十分清楚。近年来，有研究显示微小RNA（microRNA）在老年退行性骨关节炎的发病中起着关键作用。已有研究证明，对miR-140的编码基因定向敲除，小鼠可发生与年龄相关的膝关节骨性关节炎样改变，如关节软骨的纤维化、缺失蛋白多糖等[2]。为深入探究人OA及miR-140的相关性的机制，且初步分析下游靶基因的生物信息，笔者对不同分级OA患者的膝关节液进行实时荧光定量PCR检测miR-140表达水平，现报道如下。 　　1 资料与方法 　　1.1 一般资料 选择本科收治的膝关节骨性关节炎患者60例为关节炎组（OA组），按LawrenceX线诊断标准[3]：Ⅰ～Ⅳ