Survivin基因多态性与结直肠癌发病风险的相关性分析.docVIP

Survivin基因多态性与结直肠癌发病风险的相关性分析 　　【摘要】 目的：探讨Survivin基因多态性与结直肠癌发病风险的关联。方法：选取2013年1月-2015年5月本院收治的直肠癌患者186例作为研究组，同时选取健康者194例作为对照组。抽取两组患者外周血并使用蛋白酶K消化-饱和氯化钠盐析法提取DNA，采用聚合酶链式反应（PCR）和连接酶检测反应（LDR）对rs9904341C/G多态位点进行基因分型，比较两组的基因分型结果。结果：研究组C/C基因频率为44.62%，高于对照组的28.35%，C/G基因频率为39.78%，低于对照组的49.48%，比较差异均有统计学意义（P0.05）。与对照组比较，研究组Survivin C/G基因型的OR值为0.764（0.54～1.04）（P=0.020），C/C基因型的OR值为1.376（0.91～2.07）（P=0.031），G/G基因型的OR值为1.004（0.84～1.13）（P=0.121），携带C/G基因会降低患者的发病风险，而携带C/C基因则会升高发病危险。结论：Survivin基因多态性与结直肠癌发病风险有较高的关联性，为结直肠癌的诊断及探讨靶标相关miRNA多态作为结直肠癌风险个体识别的标志及其机制提供理论依据和实验基础，也意味着寻找和筛选与肿瘤发病风险密切相关的miRNA多态有望成为系统研究肿瘤早期诊断及发病机制的有效途径，因此具有广阔的实用价值和应用前景。 　　【关键词】 Survivin基因多态性； 结直肠癌； 发病风险 　　【Abstract】 Objective：To investigate the association of Survivin gene polymorphisms with the risk of colorectal cancer.Method：From January 2013 to May 2015，186 cases of rectal cancer in our hospital were selected as the research group and 194 cases of healthy persons were selected as control group at the same time.They were collected peripheral blood and extract DNA by using proteinase K digestion and saturated sodium chloride salting out method，and polymerase chain reaction （PCR），ligase detection reaction （LDR） were used for rs9904341C/G polymorphism genotyping，the genotyping results in two groups were compared.Result：The C/C 　　gene frequency was 44.62% in the research group，higher than 28.35% of the control group，C/G gene frequency was 39.78%，lower than 49.48% of the control group，the differences were statistically significant（P0.05）.Compared with the control group，in the research group，the OR value of Survivin C/G gene was 0.764（0.54-1.04）（P=0.020），the OR value of Survivin C/C gene was 1.376（0.91-2.07）（P=0.031），the OR value of Survivin G/G gene was 1.004（0.84-1.13）（P=0.121）.The risk of colorectal cancer was reduced in the group with C/G gene，while was elevated in the group with C/C gene.Conclusion：Survivin gene polymorphism and risk of colorectal cancer have a higher relevance，for the diagnosis of colorectal c