乙肝患者血清前S1抗原检测及其临床价值.docVIP

乙肝患者血清前S1抗原检测及其临床价值 　　[摘要] 目的 探讨和分析血清前S1抗原（Pres1）在乙肝感染患者临床检测中的检测方法和应用价值。方法 方便选取该院2014年3月―2015年2月所收治的乙型肝炎感染患者190例作为研究对象，通过酶联免疫吸附试验方法，检测患者的血清前S1抗原和乙肝病毒5项指标，并运用荧光定量PCR法，来检测患者的HBV-DNA指标，然后分析血清前S1抗原和HBV-DNA和HBeAg指标的阳性。结果 经检测，以HBV-DNA阳性为标准，患者血清前S1抗原和HBV-DNA检测的阳性率分别为96.9%和98.5%，患者的该两项指标对比，差异无统计学意义（χ2=0.037，P0.05）；以血清 HBsAg 指标阳性结果为标准，患者血清前S1抗原和HBeAg指标检测的阳性率分别为88.5%和96.6%，患者的该两项指标对比，差异无统计学意义（χ2=1.362，P0.05）。结论 乙肝病毒前S1抗原可以较好地反映出HBV的复制状况，对于乙肝感染者开展血清前S1抗原检测，能够明显地降低单纯血清学检验中的假阴性率，并降低误诊和漏诊的发生率，值得临床推广。 　　[关键词] 乙肝前S1抗原；乙肝病毒；乙肝感染 　　[中图分类号] R446.6 [文献标识码] A [文章编号] 1674-0742（2016）04（c）-0098-02 　　[Abstract] Objective To discuss and analyze the detection method and application value of serum pre-s1 antigen in the clinical detection of hepatitis B patients. Methods 190 cases of hepatitis B patients admitted and treated in our hospital from March 2014 to February 2015 were selected as the research objects， the serum pre-s1 antigen and 5 indexes of hepatitis B virus of patients were detected by the enzyme-linked immunosorbent assay method， the HBV-DNA index of patients was detected by the fluorescence quantitative PCR method， and then the positive rates of serum pre-s1 antigen， HBV-DNA and HBeAg indexes and correlation between them were analyzed. Results The positive rates of serum pre-s1 antigen and HBV-DNA detection were respectively 96.9% and 98.5% taking the HBV-DNA positive results as standards， and there was no obvious difference by comparison and there was no statistical significance， （χ2=0.037， P0.05）； the positive rates of serum pre-s1 antigen and HBeAg index detection of patients were respectively 88.5% and 96.6% taking the serum HBsAg index positive results as standards， and there was also no obvious difference by comparison， and there was no statistical significance，（χ2=1.362， P0.05）. Conclusion Hepatitis B virus pre-s1 antigen can better reflect the replication status of HBV， and it can obviously decrease the false negative rate of simple serologic testing and decrease