冠状动脉粥样硬化患者细胞因子信号.docVIP

冠状动脉粥样硬化患者细胞因子信号 　　[摘要] 目的 通过检测冠状动脉粥样硬化患者细胞因子信号转导抑制剂1（SOCS1）基因多态性的表达及外周血中相关细胞因子的含量，来探讨SOCS1与细胞因子及左心室射血分数的关系及其在冠状动脉粥样硬化性心脏病发生、发展中的作用及临床意义。 方法 选择2012年1月～2014年12月新疆医科大学第一附属医院收治的冠心病患者196例为研究组，选择同期196名健康志愿者作为对照组。利用聚合酶链反应及单核苷酸多态性分析两组SOCS1基因的多态性，同时利用酶联免疫吸附测定（ELISA）法检测血浆中白介素-1β（IL-1β）、肿瘤坏死因子α（TNF-α）、胱抑素C（Cys-C）、同型半胱氨酸（Hcy）的水平。 结果 研究组和对照组rrs173516427等位基因频率比较差异有统计学意义（P 0.05）。 结论 SOCS1与冠状动脉粥样硬化的发病过程有一定的相关性，可能参与其发病过程。 　　[关键词] 冠状动脉粥样硬化；细胞因子信号转导抑制剂1；细胞因子；多态性 　　[中图分类号] R541.4 [文献标识码] A [文章编号] 1673-7210（2016）03（b）-0112-05 　　[Abstract] Objective To explore the relationship of suppressor of cytokine signaling 1 （SOCS1） with cytokines and the left ventricular ejection fraction， and the role they play in the occurrence and development of coronary heart disease by detecting the expression of SOCS1 gene polymorphism and the contents of related cytokines in peripheral blood of patients with atherosclerosis of coronary artery. Methods 196 patients with coronary heart disease admitted to the First Affiliated Hospital of Xinjiang Medical University from January 2012 to December 2014 were chosen as the study group， 196 cases of healthy volunteers at the same time were chosen as control group. Polymerase chain reaction and single nucleotide polymorphism were used to analyze SOCS1 gene polymorphisms of two groups， ELISA method was used to test interleukin-1β （IL-1β）， tumor necrosis factor-α （TNF-α）， cystatin c （Cys-C） and homocysteine （Hcy） levels in plasma. Results There were significant differences in allele frequencies of rand rs173516427 between study group and control group （P 0.05）. Conclusion There is a certain relationship between the SOCS1 and pathogenesis of atherosclerosis of coronary artery， SOCS1 may take part in the pathogenesis of disease. 　　[Key words] Atherosclerosis of coronary artery； Suppressor of cytokine signaling 1； Cytokines； Polymorphism 　　随着人们生活水平的提高，心血管疾病的发病率和病死率逐年升高，并有年轻化和加速的趋势，已成为我国居民健康的“头号杀手”[1]，因此，对于心血管疾病的早期诊断和治疗是目前医务工作者面临的重要任务。动脉粥样