何鹏程修改稿.docVIP

目 录 摘要 1 关键词 1 Abstract. 1 Key words 1 1 绪论 2 1.1葛根的概述 2 1.2 国内外研究现状 3 2 葛根黄酮的提取 3 2.1 主要仪器与试剂 3 2.2 黄酮含量的测定 4 2.2.1 黄酮含量测定原理 4 2.2.2 芦丁标准溶液的制备 4 2.2.3 标准曲线的绘制 4 2.3 水浸法提取葛根黄酮 4 2.4 纤维素酶法提取葛根黄酮 4 2.5 超声辅助法提取葛根黄酮 4 2.6 微波辅助法提取葛根黄酮 5 3 结果与分析 5 3.1 传统水浸法提取葛根黄酮 5 3.1.1 料液比对葛根黄酮提取率的影响 5 3.1.2 浸提时间对葛根黄酮提取率的影响 5 3.1.3 浸提温度对葛根黄酮提取率的影响 6 3.2 纤维素酶法提取葛根黄酮 7 3.2.1 纤维素酶用量对黄酮提取率的影响 7 3.2.2 纤维素酶酶解时间对黄酮提取率的影响 7 3.3 超声辅助法提取葛根黄酮 8 3.3.1 超声功率对葛根黄酮提取率的影响 8 3.3.2 超声时间对葛根黄酮提取率的影响 9 3.4 微波辅助法提取葛根黄酮 9 4 结论 10 参考文献 10 致谢 11 葛根黄酮的提取工艺研究 何鹏程 (湖南城市学院化学工程与工艺专业2012届学生） [摘 要] 目的:建立葛根黄酮提取优选方案。方法:采用传统水浸提法、纤维素酶法、超声辅助法和微波辅助法4种不同处理方法葛根黄酮进行提取。结果:纤维素酶法较优案为料液1:40、50℃、pH 4.5，200 U/ml2 h；微波辅助法较优方案为料液比1：40、8 min预处理后，80℃下水浸提1.5h；超声辅助法较优方案为料液比1：40、300W、6 min预处理后，80℃下水浸提1.5h；传统水浸提法较优方案为料液比1：40、80℃下水浸提1.5h。结论：纤维素酶法提取葛根黄酮效果最佳，是葛根黄酮提取首选方案，传统水浸提法宜结合超声辅助法和微波辅助法。 [关键词] 葛根黄酮；传统水浸提法；纤维素酶法；超声辅助法；微波辅助法 Sdudy on Extraction of Flavanoid from Radix Pueraria He Pengcheng (2012 Year Student of Chemical Engineering and Technology Major, Hunan City University) [Abstract] Objective: To establish an optimum method of extraction flavanoid from Radix pueraria. Method: Tradition water leach, cellulose enzyme, ultrasonic assistant and microwave assistant extracting procedures were used for flavanoid extraction. Result: The optimum condition of extraction of Pueraria flavanoid was as follows: solid to liguid ratid was 1:40 with enzyme of 200 U/ml extraction for 2 hours at 50 ℃, pH 4.5 in cellulose enzyme extraetion; the third-gears microwave power was used for 8 minutes pretreatment, then extracted at 80 ℃ for 1.5 hours in the mierowave assistance extracting; the ultrasonic power 300 W and 6 minutes of pretreatment were used with solid to liquid ratio of 1:40, then extracted at 80 ℃ for 1.5 hours in the mierowave assistance extracting; and extracted at 80 ℃ for 1.5 hours with solid to liquid ratio of 1:40 in t