tables1.-plos.docVIP

Supplementary File Table 1 Primers for amplification of TCRB CDR3 by multiplex PCR Primer type Multiplex set TCRBVa Primer Sequences 5’ to 3’ Forward specific primers 20nmol/L A BV1 AGGTGACACTATAGAATA CTTGCACTCTGAACTAAACC BV2 AGGTGACACTATAGAATA TACCGTTCCCTGGACTTTC BV3 AGGTGACACTATAGAATA CAAAGTAACCCAGAGCTCG BV4 AGGTGACACTATAGAATA CCTGGACAGAGCCTGACA BV5 AGGTGACACTATAGAATA GAGWVRVARAGGAAACTTCCCT B BV6 AGGTGACACTATAGAATA RMKCTCAGGTGTGATCCAA BV7a AGGTGACACTATAGAATA AACCTTCACCTACACGCCC BV7b AGGTGACACTATAGAATA TBCCTTCACCTACACACCC BV8 AGGTGACACTATAGAATA ATGCRRGGACTGGAGTTG BV9 AGGTGACACTATAGAATA AATGAAACAGTTCCAAATCGC C BV11 AGGTGACACTATAGAATA CGAGGAATGGAACTACACC BV12a AGGTGACACTATAGAATA TGAGATGTCACCAGACTGA BV12b AGGTGACACTATAGAATA TGACGTGTCACCAGACTTG BV13 AGGTGACACTATAGAATA ACTCAGACCCCAAAATTCC BV13.5 AGGTGACACTATAGAATA ATCACCCAGGCACCAACATCT BV14 AGGTGACACTATAGAATA ATAAGGGAGATGTTCCTGAA BV15 AGGTGACACTATAGAATA ATTCTCCCTGTCCCTAGAG D BV16 AGGTGACACTATAGAATA TCAGTTCCCCAGCCACAG BV17 AGGTGACACTATAGAATA CAGAAAGGAGATATAGCTGAA BV18 AGGTGACACTATAGAATA GAGGAAGGTCTGAAATTCAT BV20 AGGTGACACTATAGAATA AGTTCATCCTGAGTTCTAAG E BV21 AGGTGACACTATAGAATA CTCTCAAGATCCAGCCTG BV22 AGGTGACACTATAGAATA CCAGACTCCCAGCCATCA BV23 AGGTGACACTATAGAATA AAATGCTATCCTATCCCTAG BV24 AGGTGACACTATAGAATA CAATGAAGCAGACACCCCT Reverse specific primer 20nmol/L TCRBCr GTACGACTCACTATAGGGA CTCAAACACAGCGACCTC Forward universal primer* 800nmol/L AGGTGACACTATAGAATA Reverse universal primer 800nmol/L GTACGACTCACTATAGGGA The forward specific primers and reverse specific primer are separately fused with universal sequences underlined matched with universal primers; a, Nomenclature from Arden et al; *, The primer is labeled by 5-Carboxyfluorescein. 1