温度与CO2浓度升高对集胞藻PCC6803修复UV损伤的关键基因转录量的影响剖析.docVIP

DOI: 10.5846/stxb201306101617 温度与CO2浓度升高对集胞藻PCC 6803 修复UV损伤的关键基因转录量的影响 顾婷婷1许敏1赵以军1, 2程凯1, 2,* ( 1.华中师范大学生命科学学院, 武汉 430079; 2.湖北工业大学, 武汉 430068 ) 摘要：通过Taqman探针绝对定量法研究了集胞藻PCC 6803在五种不同的环境条件下：(1)25℃+400μmol/mol CO2，(2)29℃+400μmol/mol CO2，(3)25℃+800μmol/mol CO2，(4)29℃+800μmol/mol CO2，(5)25℃+1200μmol/mol CO2，其phrA/psbA1/psbA2/psbA3等UV修复基因和16S rRNA基因的转录本拷贝数的变化情况。结果表明：温度与CO2浓度的升高可以导致集胞藻PCC 6803的psbA2/psbA3基因和16S rRNA转录本拷贝数的大幅减少，说明温室效应将有可能导致蓝藻的UV损伤修复能力与核糖体合成能力的下降；温度升高和CO2浓度升高对psbA2/psbA3基因和16S rRNA转录本拷贝数的联合作用表现为互相抵消，说明温度升高与CO2浓度升高的联合作用的机制较复杂，值得深入研究。 关键词：温度; CO2浓度; 转录; phrA/psbA1/psbA2/psbA3 Effects of increased CO2 and elevated temperature on transcript levels of key ultraviolet damage repair genes in Synechocystis sp. PCC 6803( GU Tingting1, XU Min1, ZHAO Yijun1, 2, CHENG Kai1,2,* 1 College of Life Science, Central China Normal University, Wuhan 430079, China 2 College of Resources and Environmental Engineering, Hubei University of Technology, Wuhan 430068, China Abstract: At the beginning of this century, the atmospheric CO2 concentration had increased by 40% compared with that in the pre-industrial era, and at the end of this century it is expected to reach 550–900 μmol/mol. The temperature is also expected to increase by 1.6(C–7.0(C. To date, much research has focused on the effects of elevated CO2 or/and elevated temperature on the growth, physiology, and ecology of cyanobacteria, but little is known about the combined effects of these factors on the ability of cyanobacteria to repair damage caused by ultraviolet (UV) radiation. In this study, Synechocystis sp. PCC6803 was grown for 15 weeks under five different conditions: (1) 25(C+ 400 μmol/mol CO2 (control group); (2) 29(C+ 400 μmol/mol CO2; (3) 25(C+800 μmol/mol CO2; (4) 29(C+ 800 μmol/mol CO2; and (5) 25(C +1200 μmol/mol CO2. Total RNA was extracted from cyanobacteria in each treatment, and was used to synthesize cDNA. Then, the transcript levels of four UV damage repair genes (phrA/psbA1/psbA2/psbA3) and th