摘要313.docVIP

以羧甲基纤维素钠为唯一碳源,从东北地区中经反复筛选及刚果红鉴定,获得一株高活力纤维素酶生产菌株C-36 。与枯草芽孢杆菌标准菌株进行了参比试验,初步鉴定该细菌为枯草芽孢杆菌。生长条件的测定显示该菌生长pH 范围较宽,在pH 710 生长最好,在pH 810～1010 的碱性条件下长势较好。摇瓶发酵粗酶液的性质测定表明,该菌所产生的内切酶在pH 710 左右酶活较高,酶活力为11280 IU/ mL。 One strain of cellulose producing bacteria which can degrade sodium carboxymethyl cellulose was isolated on the selected medium by using CMC-Na as only carbon source from the Northeast of China. The strain has high endo -β- glucanase producing ability proved by the congo red dying method on the cul2ture plates. It is identified as B acill us subili us according to it s morphological , physiological and bio2chemiscal characteristics. It is revealed by the experiment s in which C - 36 grows in a broad range of pH. While the optimum pH for it s growing is pH 710 and it also grows well in more alkaline circum2stances. Result s in shaking flask culture show that high cellulase activity (CMCase) can be detected in the condition of pH 710 and the cellulase activity is 11280 IU/ mL. 以羧甲基纤维素钠为唯一碳源,从东北地区自然环境中筛选高产纤维素酶的菌株；采用传代试验及刚果红鉴定获得19株菌体形态不同的菌株，通过摇床试验对获得的菌株进行是产酶培养，同时测定滤纸酶活，得出结果：在转速120r/min，28℃条件下，C-5在48h达到酶活峰值，为1.332U/ml；试验表明，以新华1号滤纸为底物的反应体系中，C-5的纤维素酶活直较大，即具有较强的降解纤维素能力。 One strain of cellulose producing bacteria which can degrade sodium carboxymethyl cellulose was isolated on the selected medium by using CMC-Na as only carbon source from the Northeast of China.; with passage testing and identification of access to Congo red 19 morphology of different strains, obtained by shaking test The strain was conducted fermentation culture, simultaneous determination of filter paper activity, the outcome: In the speed 120r/min, 28 ℃ condition, C-5 in the 48h to reach peak activity, in order to 1.332U/ml; tests showed that in order to Xinhua No. 1 filter paper as substrate of the reaction system, C-5 of the cellulase activity more directly, that has a strong ability to degrade cellulose. 以羧甲基纤维素钠为唯一碳源,从东北地区自然环境中筛选高产纤维素酶的菌株；采用传代试验及刚果红鉴定获得19株菌体形态不同的菌株，通过摇床试验对获得的菌株进行是产酶培养，同时测定滤纸酶