ThePredominantWT1Isoform(+KTS)EncodesaDNA-.pptVIP

The Predominant WT1 Isoform (+KTS) Encodes a DNA-Binding Protein Targeting the Planar Cell Polarity Gene Scribble in Renal Podocytes by Julie Wells, Miguel N. Rivera, Woo Jae Kim, Kristen Starbuck, and Daniel A. Haber Mol Cancer Res Volume 8(7):975-985 July 14, 2010 ?2010 by American Association for Cancer Research Confirmation of candidates from WT1 binding site screen. Julie Wells et al. Mol Cancer Res 2010;8:975-985 ?2010 by American Association for Cancer Research Localization of the WT1(+KTS) binding site within the Scribble promoter. Julie Wells et al. Mol Cancer Res 2010;8:975-985 ?2010 by American Association for Cancer Research Mutational analysis of the WT1 binding site within the Scribble promoter. Julie Wells et al. Mol Cancer Res 2010;8:975-985 ?2010 by American Association for Cancer Research Coexpression of Scribble and WT1 in mouse kidneys and cultured podocytes. Julie Wells et al. Mol Cancer Res 2010;8:975-985 ?2010 by American Association for Cancer Research WT1 regulates Scribble expression. Julie Wells et al. Mol Cancer Res 2010;8:975-985 ?2010 by American Association for Cancer Research Confirmation of candidates from WT1 binding site screen. A, reverse transcription-PCR analysis of expression levels of Wt1, Scrib, Bbs1, Imp2, Fgf18, Hs2st1, and Gapdh in RNA isolated from various mouse tissues and a podocyte cell line. B, ChIP analysis of protein binding to candidate target gene promoters in U2OS-derived cell lines 16 hours after induction of either WT1(?KTS) or WT1(+KTS). Protein binding to the Amphiregulin promoter is shown as a positive control, whereas analysis of protein binding to the Albumin control is shown as a negative control. Binding of RNA polymerase II (RNAP) and acetylated histone H3 (AcH3) were included as positive controls and IgG as a negative control. C, ChIP analysis of protein binding to the promoters for Scribble, Amphiregulin, and Dihydrofolate reductase (Dhfr) in neonatal mouse kidneys and (D) to the promoters for Sc