soybean molecular breeding.ppt

Flores T.et al. Silencing of GmFAD3 gene by siRNA leads to low a-linolenic acids (18:3) of fad3-mutant phenotype in soybean [Glycine max (Merr.)]. Transgenic Res (2008) 17:839–850 Flores T.et al. Silencing of GmFAD3 gene by siRNA leads to low a-linolenic acids (18:3) of fad3-mutant phenotype in soybean [Glycine max (Merr.)]. Transgenic Res (2008) 17:839–850 Flores T.et al. Silencing of GmFAD3 gene by siRNA leads to low a-linolenic acids (18:3) of fad3-mutant phenotype in soybean [Glycine max (Merr.)]. Transgenic Res (2008) 17:839–850 Potential genome manipulations using ZFNs. ZFN-mediated targeted genome modification relies on gap repair mechanisms 3.3.2核酶基因操作 Structural features of the zinc finger nuclease (ZFN) assembly and expression vector systems. 3.3.3 site specific recombination system (Cre-lox) two-step transformation: In the first step, a vector containing the target lox site between the promoter and cre gene is introduced into the plant genome. In the second step, the vector containing the desired gene and the lox site is re-introduced into the plant genome. When the desired gene is integrated into the target site, expression of the recombinase gene is stopped and the desired gene is stabilized at the target site. After integration,the recombinase and two selectable marker genes that are no longer needed remain at the target locus. Li Zhongsen et al. A Cre/loxP-mediated self-activating gene excision system to produce marker gene free transgenic soybean plants. Plant Mol Biol (2007) 65:329–341 3.3.4 Multigene engineering in plants Multigene transfer methods for plants Multigene transfer for metabolic engineering Vector for multigene transfer 4 Breeding by design 万建明 作物分子设计育种。作物学报，2006，32：455-462 Peleman J D and Van der Voort J R. Breeding by design. Trends Plant Sci, 2003,8:330-334 作物分子设计育种将在多层次水平上研究植物体所有成分的网络互作行为和在生长发育过程中对环境反应的动力学行为;继而使用各种“组学”数据,在计算机平台上对植物体的生长、发育和对外界反应行为进行预测;然后根据具体育种目标,构建品种设计的蓝图;最终结合育种实践培育出符合设计要求的农作物新品种。 Based on understanding the g