利用表型数据构建陆地棉核心种质.docVIP

利用表型数据构建陆地棉种质 1,2，2， 贾银华2，杜雄明2，王谧1 1长江大学农学院，荆州4340252中国农业科学院棉花研究所生物国家重点实验室，河南安阳455000） ：本研究陆地棉种质资源，根据分组成，在分组的基础上利用性状用分析陆地棉核心种质占种质资源总量的%。不同的均值t测验、方差F测验、变异系数主成分分析和评价。表明，构建的陆地棉核心种质可以代表全部种质的遗传多样性 关键词：陆地棉；核心种质；分析遗传多样性 Construction of Core Collection of Upland Cotton based on Phenotypic Data DAI Pan-hong1,2, SUN Jun-ling2, JIA Yin-hua2, DU Xiong-ming2, WANG Mi (1Agricultural College, Yangtze University, Jingzhou Hubei 434025; 2State Key Laboratory of Cotton Biology/CottonＲesearch Institute，Chinese Academy of Agricultural Sciences，Anyang Henan 455000) Abstract: total 5963 of germplasm collection of upland cotton accessions were sampled for constructing a core collection. The whole collection was firstly divided into 11 groups based on the main the traits and genotypes. The resultant core collection including 281 accessions was constructed with 21 traits in each subset by unweighted pair-group method with arithmetic means (UPGMA), which accounted for 4.71% of total collection. Furthermore the representativeness between core collection and total collections was assessed by the t test, F test of variance, coefficient of variation, the diversity index t test, average, range, phenotypic variance and variation coefficient, mean difference percentage, variance difference percentage, coincidence rate of range, changeable rate of coefficient of variation and principal component analysis. The results demonstrated that the selected core collection could represent the genetic diversity of initial collection. Key words: upland cotton; core collection; clustering analysis; genetic diversity 棉花是重要的天然纺织纤维引进中国是世界上种植面积最大的棉种，也是棉产国主栽类型产量占的%以上[1-2]，高效利用陆地棉资源现代育种服务，提高育种效率和稳步增产显得尤为重要。保存陆地棉资源712份，其中栽培种有362份[3]。如此丰富的遗传资源育种工作提供大量的材料，上能够有效开发利用的却庞大的资源种质资源的保存、研究、评价和利用带来诸多困难[4]核心种质是解决这的方法，既保证了的遗传多样性，。种质的概念Frankel[5]提出，1989年Brown[6]做了进一步完善, 其目的就是以最少的资源份数遗传重复的代表种质资源的遗传多样性等[7]核心种质还应包括具有应用价值的基因外核心种质的构建一类、、等性状数据如大麦[8]中国小豆[9]野苹果[10]新疆甜瓜42]、灰楸[43]、中国芒44]、葡萄45]等作物，、同工酶分子标