园艺植物生物第七章浅析.ppt

Genetic Engineering also called genetic modification, is the direct manipulation of an organisms genome using biotechnology.  New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or knocked out, using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add