酶的分子修饰background课程.pptVIP

1990年Goodson将干扰素的Thr3替换为Cys3，并通过PEG修饰，水溶性增加，半衰期延长(工程二硫键 ) 噬菌体T4溶菌酶Ile3突变为Cys3，使与Cys97形成二硫键，催化活性不变，但热稳定性显著提高(工程二硫键) xylanase(工程二硫键) used to treat wood pulp in paper production needs to function at high temp Glu49位于色氨酸合成酶的疏水核心部分。如Ala取代，稳定性增加(增加蛋白质内部疏水性) PEGylated microspheres: Li et al. showed significant increases in circulation time by preparing controlled release microparticles using PEG-PLGA block copolymers ? Block copolymer self-emulsifies and PEG coats both internal and external aqueous interfaces ? Benefit of improved protein stability within microspheres? Block copolymer loca