FoxM1接异构体在上皮性卵巢癌中的表达及相关功能研究.docVIP

FoxM1剪接异构体在上皮性卵巢癌中的表达及临床意义研究 [摘要] 目的：观察FoxM1剪接异构体在女性上皮性卵巢癌中的表达特征，并探讨其应用价值。方法：恶性组50例，为经手术治疗及术中获取病灶组织病理检测确诊的上皮性卵巢癌患者；良性组50例，为影像学及病理诊断为卵巢良性肿瘤者；健康组50例，为其它妇科疾病入院但经临床症状、影像学检查等未见卵巢异常者。均取卵巢病理组织切片免疫组化染色，免疫荧光法检测FoxM1表达阳性情况。结果：恶性、良性和健康组卵巢组织FoxM1的表达阳性率比较差异具有统计学意义（X2=62.632，P0.01），两两比较，恶性组显著高于良性组及健康组（X2=27.473，60.864，P0.01），良性组显著高于健康组（X2=10.187，P0.01）。不同临床分期的上皮性卵巢癌样本FoxM1的表达差异具有显著性，I、II期病变FoxM1阳性率显著低于III、IV期（X2=5.882，P0.05）。但浆液性乳头状囊腺癌FoxM1阳性率与非浆液性乳头状囊腺癌比较差异无显著性（X2=0.039，P0.01）。恶性肿瘤患者随着FoxM1表达增强，患者的3年生存率（OS）和无进展生存率（PFS）逐渐下降。结论：FoxM1剪接异构体在上皮性卵巢癌中呈现为高表达，且随着临床分期的增高FoxM1的阳性率提高，随FoxM1表达增高恶性肿瘤患者的生存率下降，FoxM1可能参与卵巢癌的发生与进展过程，其机制可能与FoxM1的细胞周期调控作用有关。 [关键词] FoxM1剪接异构体，上皮性卵巢癌，基因表达，细胞周期 The expression and clinical significance study of FoxM1 isoforms in epithelial ovarian cancer [Abstract] Objective: To observe expression features of FoxM1 isoform in women with epithelial ovarian cancer, and to explore its value. Methods: 50 cases of malignant group, were the patients treated by surgery and got intraoperative detection of pathological lesions diagnosed epithelial ovarian cancer; 50 cases of benign group, were patients of diagnostic imaging and pathological ovarian benign tumor; 50 cases of healthy group, were patients admission but for other gynecological diseases by clinical symptoms, imaging studies, such as no ovarian abnormalities. Ovarian pathology tissue sections were taken immunohistochemistry, FoxM1 expression positive situation was detected by immunofluorescence. Results: The difference of FoxM1 ovarian tissue expression rate of malignant, benign and healthy group was statistically significant (X2= 62.632, P 0.01), pairwise comparison, the malignant group was significantly higher than that of benign group and the healthy group (X2=27.473, 60.864, P0.01), the benign group was significantly higher than the healthy group (X2 = 10.187, P0.01). The difference of expression in epithelial ovarian cancer samples FoxM1 of different clinical stages was significant, the FoxM1 positive rate of