气-质联用仪的基本结构及工作原理解剖.ppt

* * Future papers will address the application of other ionization techniques besides EI. The spectrum shown above is the NIST library spectrum for Chlorothalonil in EI Full Scan. The next slide shows the spectrum produced using PPINICI on the DSQ with methane as the reagent gas. By looking at the functional groups of this pesticide what do you think the results would be? The molecular ion is 264 m/z. * The results showed what we would expect: NCI: molecular ion M = 264 m/z PCI: M+1 (264 +1) = 265 m/z and the adduct ions: M+29(264+29) = 293 m/z and M+41 (264+41) = 305 m/z An adduct ion is formed by the joining together of two species, usually an ion and a molecule, to form an ion containing all the constituent atoms of one species as well as an additional atom or atoms. Adduct ion formation most often occurs within the ion source. For example, in methane chemical ionization (CI), the C2H5+ C3H5+reagent gas ions (m/z 29 and 41) often adds to an analyte molecule [M] to form the adduct ions [M+C2H5]+ ([M+29]+) and [M + C3H5]+ ([M+41]+). * * * * * * * * * * * The TRACE DSQ incorporates a new curved quadrupole pre-filter to help eliminate the non-chemical noise associated with the production of excited neutrals in the source. In on axis designs these excited neutral can impact the detector producing excess background noise which can limit the dynamic range and sensitivity of the instrument. By employing a curverd pre-filter, The TRACE DSQ eliminates the migration of these excited neutrals and thus reduces the overall noise in the system * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * MRM方法的开发过程 步骤2 根据步骤1的结果编辑一个子离子扫描的方法。调查在不同的碰撞池电压下所得的子离子扫描图。选择丰度高的特征子离子作为MRM 方法的子离子。（一般一个母离子对应两个以上子离子） 三重四极杆质量分析器 三重四极杆质量分析器质量范围上限有1000～4000 u不同档次(也有低于l 000 u的)，分辨率大于1 M，质量精度在0．1 u。不同型号仪器结构上的区别，在于两个质量分析器连接方式不同，多是同轴直线串接的，有的是中间四极弯成90。或180。与单四极分析器一样，三重四极质量分析器，多配置EI和正、负CI离子源 （2）、离子阱质谱-时间串联 利用离子储存技术