methods checklist fecal coliforms（方法清单粪大肠杆菌群）.docVIP

APPENDIX D Methods Checklist: Fecal Coliforms-Membrane Filtration Date: Permit Holder: Township: County: NPDES Permit #: Person Interviewed: Title: Introduction: Fecal coliform bacteria are part of the coliform group of bacteria defined as gram-negative, non-spore forming rods that ferment lactose in 24 ( 2 hours at 44.5(C. Lactose can be detected through the production of gas in a multiple tube technique or by the acidity forming blue colonies in a membrane filter procedure. The membrane filter procedure is used to identify and enumerate coliform bacteria of fecal origin from those derived from other sources. With this procedure, an appropriate volume of water sample (or dilution) passes through the membrane filter, which retain the bacteria present in the sample. The membrane filter is then placed on an absorbent pad saturated with M-FC agar in an appropriate container. The container is incubated at 44.5(C for 24 hours, and typical blue colonies are counted to determine the number of fecal coliforms, per unit volume of sample. Although somewhat susceptible to interferences, including high solid levels and high levels of noncoliform bacteria, advantages of the membrane filter procedure include rapidity and a generally enhanced precision. 40 CFR Part 136, July 1, 1996, References: 1978 EPA Methods Manual entitled “Microbiological Methods for Monitoring the Environment, Waters, and Wastes,” (p. 124). “Standard Methods for the Examination of Water and Wastewater,” 18th Edition, 1992, Method 9222 D, (p .9-60). Table II, 40 CFR Part 136, July 1, 1996: Holding Time: 6 hours Preservation: 4(C; 0.008% Na2S2O3 Sample Container: Glass or Plastic Equipment: Incubator: Media: Filter: (Pore size): Sterilization Equipment: Required by the 18th Edition of Standard Methods (Page): Yes No NA 1. Is the laboratory equipment decontaminated via UV sterilization, ___ ___ ___ flowing steam, or boiling