芝麻粕降解菌的筛选与发酵条件优化..docVIP

芝麻粕降解菌的筛选与发酵条件优化 芝麻粕降解菌的筛选与发酵条件优化(13700字) 摘 要：从浏阳河边土壤中筛选出一株能够降解芝麻粕的菌种，并对其进行了形态学分析菌株为芽孢杆菌属。并运用现代微生物发酵技术，用其对芝麻粕进行发酵，并对菌种通过筛选得到优势菌进行培养的单因素培养和正交优 ... p芝麻粕降解菌的筛选与发酵条件优化(字)br / 摘nbsp; 要：从浏阳河边土壤中筛选出一株能够降解芝麻粕的菌种，并对其进行了形态学分析菌株为芽孢杆菌属。并运用现代微生物发酵技术，用其对芝麻粕进行发酵，并对菌种通过筛选得到优势菌进行培养的单因素培养和正交优化，确定了优势菌在本实验条件下的最佳培养基成分及最佳培养条件为：可溶性淀粉1%，芝麻粕4%，磷酸二氢钾0.2%， 氯化钙0.1%，氯化镁0.05% ，温度37 ℃，初始pH 5，接种量为5%，转速200 rpm，培养时间为48 h。在此条件下，测得蛋白酶活力可达到1236.48U/mL，生物量约为12.0times;108nbsp; cfu/mL, 通过凯氏定氮可得在最佳条件的发酵下芝麻粕的最大降解率可达到49.3%。br / 关键词：芝麻粕；芽孢杆菌；降解；蛋白酶br / br / Screening of a strain degrading sesame meal and optimization of fermentationrsquo;s conditionnbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; br / Abstract: Bacillies sp M. a strain was ablcto degerate prude poctein in sesame meal, was screened from soil near LiuYang river by media of sesame meal. The fermentation condition has been optimized by including the components of medium and the fermentation condition.The results showed that the best components of medium were the soluble starch of 1%, sesame meal of 8%,calcium chloride of 0.1%, magnesium chloride of 0.05% potassium dihydrogen phosphate ofnbsp; 0.2% and the most suitable condions were the temperature of 37℃, pH 5, inoculation volume of 5% ,rotation speed of 200 rpm, cultured for 48 h. protrase activity reached 1236.48U/mL, biomass is about 12.0 times; 108 / mL. In protein degradation process the sesame meal degradation is 49.3% in protein degradation product. br / 2.2.3 培养条件优化nbsp;nbsp;nbsp; 12br / 2.2.4 凯氏定氮nbsp;nbsp;nbsp; 12br / 3 结果与分析nbsp;nbsp;nbsp; 13br / 3.1 菌株的筛选nbsp;nbsp;nbsp; 13br / 3.2 发酵液酶活力nbsp;nbsp;nbsp; 13br / 3.3 发酵培养基成分优化nbsp;nbsp;nbsp; 14br / 3.3.1 碳源对菌株生长及酶活nbsp;nbsp;nbsp; 14br / 3.3.2 氮源对菌株生长及酶活nbsp;nbsp;nbsp; 15br / 3.3.3 金属及无机盐离子对菌株生长及酶活nbsp;nbsp;nbsp; 16br / 3.3.4 培养基成分对菌株生长及酶活影响正交试验nbsp;nbsp;nbsp; 17br / 3.4 培养条件优化nbsp;nbsp;nbsp; 18br