ppt课件-peptidemassfingerprintingforproteinidentification-hsls.pptVIP

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * Not all peaks are matched –why? Theoretical peptide list peptides lengths vs. MS range Enzyme – missed/non-specific cleavage Incorrect ORF Amino acid substitutions Ion suppression/efficiency Experimental peptide list Contaminants Trypsin autolysis peptides Hair, skin keratins Matrix molecules, clusters Unknown contaminants Modifications PTM’s – known and unknown, biological origin Oxidized methionines, – gel induced artifacts Chemical – cysteine carbamidomethylation, sample handling introduc