培养条件阴沟肠杆菌乐果降解效能的影响.docVIP

培养条件对阴沟肠杆菌乐果降解效能的影响( 官雪芳1，2刘波1*林斌2,林抗美1马丽娜1 （1福建省农业科学院生物资源研究所，福建 福州3500032．福建省农科院农业工程技术研究所，福建 福州350003）摘要： 从福建省福农生化有限公司排污口的土壤中筛选到一株对乐果有较好降解效果的菌株阴沟肠杆菌，为了使该菌能更好的应用于乐果残留污染的治理当中，特正交试验法L16（44×23），结果为：菌降解乐果的最佳发酵条件组合为处理5碳源：蔗糖、离子：CuCl2:(10 Mmol·L)、乐果浓度：100 mg·L、培养时间36 h、培养温度30 ℃、pH值8、氮源：酵母3 g·L分析各类因子对菌降解乐果的影响，得出离子对其作用影响最为显著，pH值、培养温度、乐果浓度、培养时间等也依次对菌降解乐果有较大的影响。 关键词：阴沟肠杆菌 乐果 降解 优化 正交试验 Effect of culture conditions on Enterobacter closcae degrading dimethoate GUAN Xue-fang1，2,LIU Bo1*LIN Bin2,LIN Kang-mei1, MA Lina1 (1 Agricultural Bioresources Research Institute Fujian Academy of Agricultural Sciences, Fuzhou,350003, China, 2Institute of Agricultural Engineering Technology; Fujian Academy of Agricultural Sciences, Fuzhou,350003, China ) Abstrat: By using selective cultural media, Enterobacter closcae was isolated to degrade dimethoate from the soil around the pesticide factory. In order to make it better used with residual dimethoate, the L16（44*23）orthogonal test was applicated to study the effective factors of the degradation to dimethoate of E. closcae. The results showed that the conformable carbon source for the degradation was sucrose, the ion was Cu2+ with the density of 10 Mmol/L, the density of dimethoate was 100 mg/L, the optimum temperature was 30 ℃, the initial pH was 8.0, the conformable nitrogen source was yeast extract with the density of 3 g/L, and the culture time was for 36 h with the degrading rates of 58.90%, Meanwhile,using the DPS Date Processing System, the single factor effect and sample were statisticed. The effect of ion has the most significant impact on the degradation of rates, and then followed by pH, culture time, density of dimethoate, temperature and carbon source. Key Words: Enterobacter closcae, Dimethoate, Degradation, Optimize orthogonal experiments 有机磷农药一直是我国用量最大的农药种类，每年都在十万吨以上，它在给农业生产带来效益的同时也给人类带来了很大的污染问题，大量的这类农药施于农田后，只有大约1%作用于靶标生物，其余的或残留于土壤，或通过径流进入水域，影响土壤和水体中的生物，残留于土壤、水源、大气以及农作物中，这对人类及其环境产生深远的影响[1-4]。微生物是导致环境中有机磷农药降解的方式，农药微生物降解在农