精液分析仪与传统检测法的对比.docVIP

精液分析仪与传统检测法的对比 【摘要】 目的：对传统的人工精液常规与精液质量分析仪（sperm quality analyzer, SQAV）分析的精液结果进行比较。方法：对50例精液标本采用人工精液常规分析与SQAV分别进行检测。结果：在检测精子密度、精子活率及A+B级精子活力百分率的结果上，精液质量分析仪有较好的重复性(其CV分别为1.63%、4.9%、5.76%)，且与人工精液常规分析的结果有高度相关性，在统计学上差异无显著性(Plt;0.001)。结论：在精液常规分析中，传统人工精液常规分析与SQAV具有较好的一致性，且SQA所特有的指标SMI值更能综合地反映精子质量，也是常规精液分析所无法获取的数值，故SQA对精液质量的分析在临床上诊断上具有一定的价值。 【关键词】 精液质量分析 对比 精子活力指数 　　Comparison of Sperm Analyzer and Traditional Analysis Method 　 Abstract： Objective To compare the results analyzed by traditional handmade sperm routine with the results analyzed by sperm quality analyzer(SQAV). Methods Fifty sperm samples were analyzed through handmade sperm routine and sperm quality analyzer(SQAV) respectively. Results Sperm quality analyzer(SQAV) was of good reproductive about the results of analyzed sperm density and sperm activity rate and percentage of A+B level sperm activity(the coefficient of variation values of these were 1.63%,4.9% and 5.76% respectively). There was a very good correlation between the results of two methods.the statistics showed that there was no significant difference(Plt;0.001). Conclusion Traditional handmade sperm routine and sperm quality analyzer(SQAV) were of good consistency in the sperm routine analysis,and the especial values of SMI of SQA not only reflected comprehensively sperm quality but also obtained the values which routine sperm analysis could not test,consequently,the analysis of sperm quality by SQA was of certain value in clinical diagnosis. Key words： Sperm quality analyzer； Comparison; Sperm activity index 精液质量分析是评价男性生育能力的重要检测项目，传统的人工分析方法是用改良牛鲍氏细胞计数板进行检测,其应用于精液常规分析已有半个多世纪，迄今仍为多数男性病实验室所采用，由于检测结果的差异较大，重复性较差并且缺少客观性，给临床男性不育症的诊治和科研工作带来一定的困难。目前，我院引进了以色列SQAV全自动精子质量分析仪（sperm quality analyzer, SQAV），采用现代技术对精液各种参数进行客观检测，并通过精子活力指数（SMI）对精液质量进行综合评价，具有直观、客观、快速等优点。本文采用SQA与人工检测结果进行了比较。 　　1 材料与方法 　　1.1 材料 　　1.1.1 标本来源 　　本院泌尿外科患者(不包括无精症患者)的精液,年龄17岁～40岁,剔除无活动精子的精液样本后随机抽取50例进行SQA与人工检测。 　　1.1.2 仪器 　　SQAV型