苦参碱对肝卵圆细胞增殖模型大鼠notch1, jagged1 mrna表达的影响.docVIP

苦参碱对肝卵圆细胞增殖模型大鼠Notch1, Jagged1 mRNA表达的影响 【关键词】 苦参碱；肝；卵母细胞；大鼠；Thy 　　Effect of matrine on expressions of Notch1, Jagged1 mRNA in hepatic oval cell proliferation model rats 　　【Abstract】 AIM: To study the expressions of Notch1, Jagged1 mRNA in hepatic oval cell proliferation model rats and the effect of matrine on them. METHODS: Fortyeight male SD rats were randomly separated into 4 groups according to body mass: model group(group A),lowdose matrine group( group B, 5 mg/100g), highdose matrine group (group C, 25 mg/100 g), control group (group D). The expression of Thy1 protein was detected by enzymehistochemisty, and the expressions of Notch1, Jagged1 mRNA were detected by semiquantitative RTPCR method. RESULTS: Compared with group D ，the expressions of Thy1 protein, Notch1 mRNA, Jagged1 mRNA in group A were singnificantly increased （Plt;0.01）. Compared with group A,the expressions of Thy1 protein, Notch1 mRNA,Jagged1 mRNA in group C were singnificantly increased（Plt;0.01）. CONCLUSION: Matrine could regulate Notch signaling, decrease the expression of Thy1 protein,which might be one of the mechanisms of inhibiting the proliferation of hepatic oval cells and inducing rat hepatic oval cells to differentiate into mature hepatocytes. 　　【Keywords】 matrine; liver; oocytes; rats; Thy1; Notch1 　　【摘要】 目的： 研究肝卵圆细胞增殖模型大鼠Notch1, Jagged1 mRNA表达的变化及苦参碱对其的影响. 方法： 雄性SD大鼠48只按体重随机分为4组： 模型组（A组）；苦参碱小剂量（B组）；苦参碱组大剂量(C组)；D组即正常对照组. 用免疫组化方法观察造血干细胞标记(Thy1)表达的变化、半定量RTPCR方法观察肝组织中Notch1, Jagged1 mRNA表达的变化. 结果： 与D组比较，A组Thy1蛋白, Notch1mRNA, Jagged1 mRNA表达显著升高（Plt;0.01）,与A组比较,C组Thy1蛋白, Notch1mRNA, Jagged1 mRNA表达显著下调（Plt;0.01）. 结论： 苦参碱可有效的调节Notch信号通路、下调Thy1蛋白表达，可能是其抑制卵圆细胞过度增殖、诱导卵圆细胞向肝细胞方向定向分化的机制之一. 　　【关键词】 苦参碱；肝；卵母细胞；大鼠；Thy1；Notch1 　　0引言 　　卵圆细胞作为肝脏的干细胞在肝细胞损伤修复及癌变过程中起重要作用，但目前关于卵圆细胞的来源、特性、分化增殖的调控机制尚不十分清楚. 研究证实多种细胞因子参与了卵圆细胞分化发育的调节过程［1-2］. 本实验建立了大鼠肝卵圆细胞增殖模型，观察了卵圆细胞在肝组织内的分布及Notch1, Jagged1 mRNA在该模型中的表达情况，并探讨了苦参碱对其的作用. 　　1材料和方法 　　1.1材料苦参碱标准品购自中国药品生物制品检定所，二乙酰氨