不同品种松树皮活性成分含量及其提取影响因素.docVIP

不同品种松树皮活性成分及其提取影响因素* 吴杰1 关阳1 刘跃芹1 赵雪松1 于立梅* 孟凡杰3 (1玉米深加工国家工程研究中心, 长春 130033；2仲恺农业工程学院轻工与食品学院 广州 510225±6.31 mg /g、81.1±4.67 mg /g、36.8±2.31 mg /g和24.9±3.17 mg /g。通过马尾松树皮原花青素清除DPPH自由基能力，乙醇浓度为0%，提取温度，提取时间min，液料比251。ctive Components of Different Varieties of Pine bark and Extraction Factors* WU Jie, YU Li-mei, (1National Engineering Research Center of Corn Deep Processing，Changchun 130033,China ;2 College of Light Industry and Food Science，Zhongkai University of Agricultural and Engineering， Guangzhou 510225，ive pine varieties in Southern China area was chosen and the active ingredient content and antioxidant activities were investigated to get best pine species. The effect of different extraction conditions (extraction time, extraction temperature, solvent concentration, solid-liquid ratio and extraction times)on extracting rate and free radical scavenging activity of procyanidins was also studied. The results show that: different varieties of pine bark exhibited the different content and its activity significantly. Total polyphenol content, total flavonoid content, procyanidins and water-soluble polysaccharide content from massoniana pine bark is the highest, respectively 107.5 ± 6.31, 81.1 ± 4.67 36.8 ± 2.31 and 24.9 ± 3.17 mg /g. Through the pine bark procyanidins content and DPPH radical scavenging capacity based on the composite response analysis, the effects of different extraction conditions had its remarkable, the optimum extraction conditions is 60% ethanol concentration, extraction temperature 50 ℃, extraction time 90min, ratio of liquid and material 25:1. Key words: Pine tree; active content ; extraction conditions ;antioxidant activities 中国松科(Pinaceae)中的一些松属(Pinus)植物，是组成我国大面积针叶林的重要树种，也是林业经营的主要树种。中国的松属植物有22种10个变种，几乎分布于全国各地，此外，还有从国外引种的16个种2个变种 [1-2]。现代药理学研究表明，松树皮提取物富含原花青素，具有抗氧化、降低毛细血管通透性、抑制酶活性、保护肝脏、抗病毒、抗真菌、抗溃疡、抗肿瘤、抗突变、降血脂、降血压等功效[3-6]，现已在临床医学、化妆品等领域得到应用1 材料与方法 1.1 主要材料与试剂 松树皮粉：将树皮于50下烘2h，去除木质纤维部分，以中草药粉碎机打碎成20～40目左右的树皮粉，封口袋密封保存。95%乙醇、均为分析纯1.2 主要实验仪器 恒温水浴摇