theoncogeneticroleofmicrorna-31asapotential.ppt

The oncogenetic role of microRNA-31 as a potential biomarker in oesophageal squamous cell carcinoma by Tengfei Zhang, Qiming Wang, Dan Zhao, Yaling Cui, Bangrong Cao, Liping Guo, and Shih?Hsin Lu Clin. Sci. Volume 121(10):437-447 November 1, 2011 ?2011 by Portland Press Ltd miR-31 expression in ESCC tissue and serum samples The number of cases for each group is indicated below the x-axis. Tengfei Zhang et al. Clin. Sci. 2011;121:437-447 ?2011 by Portland Press Ltd The role of serum miR-31 in the diagnosis and prognosis of ESCC (A) ROC curve analysis using serum miR-31 levels for discriminating ESCC in discovery group [AUC, 0.902 (95% CI, 0.857–0.936); cut-off value is 0.0054; sensitivity, 86.7%; specificity, 84.3%]. Tengfei Zhang et al. Clin. Sci. 2011;121:437-447 ?2011 by Portland Press Ltd Colony formation, migration and invasion analyses of three ESCC cell lines after up-regulation or down-regulation of miR-31 (A) Colony formation of the EC9706 cell line. Tengfei Zhang et al. Clin. Sci. 2011;121:437-447 ?2011 by Portland Press Ltd Detection of target genes regulated by miR-31 in the EC9706 cell line (A) Luciferase activities after transfection with the ten wild-type 3′-UTR-clone reporter constructs (Wt3′UTR) and miR-31 or NC. (B) miR-31-binding sites in the 3′-UTR region of EMP1, KSR... Tengfei Zhang et al. Clin. Sci. 2011;121:437-447 ?2011 by Portland Press Ltd miR-31 expression in ESCC tissue and serum samples The number of cases for each group is indicated below the x-axis. In the box plots, the lines denote the 10th, 25th, median, 75th and 90th percentiles for each. (A) miR-31 expression in paired ESCC cancerous and surrounding normal tissues, normalized by 2?ΔCt. (B) Relative expression of miR-31 in tissues grouped by total TNM stage, normalized by 2?ΔΔCt. (C) Expressions of serum miR-31 in healthy subjects and ESCC patients grouped by TNM stage. (D) Levels of miR-31 in serum of ESCC patients before (pre-op) and 7?days after (7?days post-op) surgical rese