Diagnostic microbiology lecture 5 Pseudomonas Abed 诊断微生物学讲座5假单胞菌床.pptVIP

Diagnostic microbiologylecture: 5PseudomonasAbed ElKader Elottol MSc. Microbiology2010 Pathogenic species 1. Pseudomonas aeruginosa 2. Ps. pseudomallei 3. Ps. mallei Pseudomonas aeruginosa May grow in : ? Disinfectants ? Contact lens solutions ? Medicinal solutions ? Humidifiers of respirators GENERAL CHARACTERISTICS 1. Obligate aerobic, gram negative bacilli indistinguishable from Enterobacteriaceae. 2. Non-lactose fermenter 3. Oxidase positive 4. Glucose oxidizer 5. Catalase positive 6. Citrate positive 7. Motile by a polar flagella 8. Produces exotoxin A = Inhibits protein synthesis and produces tissue necrosis.9. Produces collagenase which hydrolysis collagen10. produces elastase which hydrolysis elastin and is used by the organism for invasion.11. It produces pigments11.1 pyocyanin = blue green pigment11.2 pyoveridin = Yellow- fluorescence. PATHOGENICITY Ps. aeruginosa is an opportunistic pathogen that infect immunocompromized patient. Usually causes hard to treat nosocomial infections. It show resistance to most antibiotics. DISEASES caused by Ps. aeruginosa include: 1. Urinary tract infection (UTI) 6. Sepsis 2. Otitis media 7. Burn infection 3. Wound infection 8. Meningitis 4. Sinus infection 9. Endocarditis 5. Bronchopneumonia IDENTIFICATION 1. Gram staining: Ps. aeruginosa is indistinguishable from other gram negative bacilli. Therefore there is a little significance for gram staining. 2.Culture: Ps. aeruginosa grow well on ordinary media such as Blood Agar, Nutrient Agar and MacConkey Agar. = Colonies are 2-4 mm in diameter, slightly convex, grayish the smell like grape and are often hemolytic. = On Nutrient Agar, Ps. aeruginosa is easily recognized by the diffused blue-green pigmentation. Biochemical tests Serological typing: This is performed through an agglutination test based on the reactivity of somatic O antigens of intact cell with sp