不同寄主来源的灰葡萄孢对番茄的致病力分化研究.docVIP

不同寄主来源的灰葡萄孢对番茄的致病力分化研究 李喜玲 高智谋* 李艳梅 曹中革 陈伟 汪世军( 安徽农业大学植物保护学院 合肥 230036 摘 要：从合肥、蚌埠、长丰、和县等市、县的番茄、辣椒、草莓、葡萄等发病寄主上分离鉴定获得18个灰葡萄孢菌株，采用菌丝块创伤接种法，分别测定了上述不同寄主来源的灰葡萄孢菌对番茄果实和叶片的致病力。结果表明，所有供试菌株接种番茄果实后均引起发病，但不同菌株所致病斑的平均直径有显著差异，显示灰葡萄孢菌株间对番茄果实的致病力存在明显分化。按照在番茄果实上所致病斑的平均直径大小可将供试菌株致病力划分为较强、中等和较弱3种类型。总体来说，来自番茄的菌株对番茄果实的致病力较强，来自草莓、葡萄和辣椒的菌株对番茄果实的致病力较弱，但来自相同寄主的菌株间致病力也存在差异，菌株致病力差异与菌株地域来源无明显相关。供试灰葡萄孢菌株接种番茄叶片后，除CF1外，均可引起番茄叶片发病，但不同菌株所致番茄叶片病斑的平均直径也有显著差异；供试菌株对番茄叶片的致病力差异与菌株的寄主和地域来源无显著相关。 番茄灰霉病病斑平均直径致病类型地域来源 Differentiation of pathogenicity of Botrytis cinerea strains from different hosts to tomato L Xi-Ling GAO Zhi-Mou( LI Yan-Mei CAO Zhong-Ge CHEN Wei WANG Shi-Jun College of Plant Protection, Anhui Agricultural University, Hefei 230036, China Abstract: Eighteen isolates of Botrytis cinerea were obtained from the diseased plant tissue collected in Hefei, Bengbu, Changfeng and Hexian in Anhui province, by means of tissue isolating method. The pathogenicity of the isolates of B. cinerea from different hosts to the fruits and leaves of tomato were investigated by applying wound inoculation with mycelial blocks. The results showed that all of the tested isolates caused grey mould on tomato fruits, but there was significant difference in the average diameters of the lesions caused by different isolates, suggesting that there was significant differentiation in pathogenicity of B. cinerea strains to tomato fruits among isolates. According to the average diameters of the lesions on tomato fruits, the pathogenicity of the all isolates was classified into three categories: strong, intermediate and weak. In general, the isolates from tomato were more strongly pathogenic to tomato fruits than the isolates from strawberry, grape and capsicum. However, there was difference in pathogenicity among the different isolates from the same host, and the pathogenicity difference was not obviously related to the localities of isolates. After inoculating of tomato leaves, all of the tested isolates