树莓叶片生长过程中内源激素含量变化初步研究.docVIP

树莓叶片生长过程中内源激素含量变化初步研究 　　摘要：以树莓（Rubus idaeus L.）品种费尔杜德（R. idaeus L.cv. Fertod Zamatos）和哈瑞太兹（R. idaeus L.cv. Herytage）为试验材料，测定其叶片在生长过程中植物生长素（IAA）、赤霉素（GA3）和脱落酸（ABA）3种内源激素含量的变化趋势，并在2个品种间做出比较，分析3种内源激素比值及相关性。结果表明，树莓叶片从展叶到脱落过程中，内源激素含量变化趋势基本一致， GA3含量变化呈现“上升-下降-上升-下降”的变化趋势，IAA含量变化呈“先上升后下降” 的变化趋势，ABA含量变化不大，整体上为“下降-上升-下降-上升” 的变化趋势。GA3/ABA和IAA/ABA比值变化呈“先上升后下降”的变化趋势，其中哈瑞太慈叶片中GA3/ABA和IAA/ABA比值在果实采收期出现回升，后期下降，且GA3、IAA与ABA含量的相关性呈负相关。GA3/IAA比值前期无变化，进入衰老期有增长，GA3和IAA含量呈正相关。GA3和IAA含量在叶面积迅速增长时期达到最大值，而ABA在叶片进入衰老期则含量较高。 　　关键词：树莓（Rubus idaeus L.）；叶片；内源激素；含量；变化 　　中图分类号：S663.2 文献标识码：A 文章编号：0439-8114（2015）24-6280-04 　　DOI：10.14088/j.cnki.issn0439-8114.2015.24.047 　　Abstract：With Rubus idaeus L.cv. Fertod Zamatos and R. idaeus L.cv. Heritage as the experimental materials， the changing tendency of endogenous hormone content of raspberry leaves including GA3， ABA and IAA was determined to make a comparison between the two species， analysis of the ratio and correlation of the three endogenous hormones. The results showed that the changing tendency of the endogenous hormone content in raspberry basal leaves from unfolding to shedding was consistent. The change of GA3 content presented an ‘up-down-up-down’ trend； the change of IAA content was ‘first increased and then decreased’ trend， the content of ABA showed a ‘down-up-down-up’ trend. GA3/ABA and IAA/ABA ratio increased firstly and then decreased， GA3/ABA and IAA/ABA ratio of R. idaeus L.cv. Heritage leaves increased in fruit harvest period and then decreased in later stage. GA3 and IAA content was negatively correlated which ABA. GA3 /IAA ratio did not change in earlier stage， while increased in the aging period. GA3 and IAA content was positively correlated. In rapid growth period of leaf area， GA3 and IAA content reached to the maximum； while ABA content was higher in the senescence stage. 　　Key words：raspberry（Rubus idaeus L.）；leave；endogenous hormone；content；changes 　　树莓（Rubus idaeus L.）又称覆盆子、刺莓、马林等，属蔷薇科（Rosaceae）悬钩子属（Rubus spp.）