模拟酸雨及苔藓植物化感条件下紫苏种子萌发特性的研究.docVIP

模拟酸雨及苔藓植物化感条件下紫苏种子萌发特性的研究 　　摘要：通过模拟酸雨胁迫及苔藓化感作用两种不同的生长环境，初步探讨了模拟酸雨及3种苔藓凤尾藓（Fissidens bryoides）、提灯藓（Mnium hornum）、缩叶藓（Ptychomitrium）种植水对紫苏（Perilla frutescens）种子萌发特性的影响。结果表明，pH为5.0及以上的低浓度模拟酸雨环境对紫苏种子的萌发及幼苗生长影响不大，但随着酸化程度的加深，对紫苏种子萌发的抑制作用明显增加，当pH降到1.0时种子不发芽。紫苏幼苗叶片POD活力随胁迫时间的延长呈先升后降的趋势。而叶片MDA含量除pH为5.0处理组与对照组相近外，其他胁迫浓度下均有显著增加，说明pH低于5.0紫苏幼苗生长受到较严重损害。在苔藓种植水培养环境中，与对照组相比，紫苏种子发芽率、发芽指数、活力指数均随苔藓植物种植水浓度的升高而升高，在浓度为100%时对种子萌发的促进效果最为显著。 　　关键词：紫苏（Perilla frutescens）；种子萌发；生理指标；酸雨；化感作用 　　中图分类号：Q949.777.6；Q945.79 文献标识码：A 文章编号：0439-8114（2012）23-5336-04 　　Study on Germination Characteristics of Perilla frutescens under Simulation Environment of Acid Rain and Bryophyta 　　XIE Tian，JIANG An-na，WANG Man-jun，YAN Feng，FANG Yuan-ping，XIANG Jun 　　（Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization / College of Chemisitry and Life Sciences， Huanggang Normal University， Huanggang 438000，Hubei，China） 　　Abstract： By simulating two different growth environment， acid rain stress and moss allelopathy， the effects of acid rain and three bryophyte cultivate water （Fissidens bryoides，Mnium hornum，Ptychomitrium） on Perilla frutescens seed germination was studied. The results showed that the simulated acid rain with pH lower than 5.0 had little effect on P. frutescens seed germination and seedling growth； as the increasing of acidification， there was a significant inhibition of seed germination， and the seed could not germinate under the pH lower than 1.0. The POD activity in P. frutescens blade increased firstly and then decreased as the stress time prolonged， and the MDA content was decreased when pH was lower than 5.0， suggesting that P. frutescens seedling growth was affected by pH lower than 5.0. In the culture water environment， compared to the control， P. frutescens germination rate， germination index， vigor index increased as the bryophyte planting concentration increasing， and the treatment with the most significant effect was 100% bryophyte plant