Green Fluorescent Protein绿色荧光蛋白.pptVIP

Green Fluorescent Protein a B/MB senior seminar brought to you by Colm O’Carroll This presentation will cover The structural aspects of GFP which make fluorescence possible The advantages of using GFP and GFP mutants over other fluorescent markers The use of GFP to monitor viral movement in plants The Green Fluorescent Protein GFP’s unique structure Composed of 238 amino acids “Paint in a can” Each monomer composed of a central ?-helix surrounded by an eleven stranded cylinder of anti-parallel ?-sheets Cylinder has a diameter of about 30A and is about 40A long Fluorophore located on centr