肿瘤细胞DNA甲基化检测与方法新进展概述.pdf

网络出版时间：2016-04-29 10:21:10 网络出版地址：/kcms/detail/31.1600.Q1021.013.html 第28卷第4期 生命科学 Vol. 28, No. 4 2016年4月 Chinese Bulletin of Life Sciences Apr., 2016 DOI: 10.13376/j.cbls/2016066 文章编号：1004-0374(2016)04-0513-08 ? 技术与应用 ? 肿瘤细胞DNA 甲基化检测技术与研究方法新进展 赖伊杰，康亚妮* (上海交通大学生物医学工程学院，上海 200240) 摘　要 ：DNA 甲基化作为一种常见的表观遗传学修饰方式，在基因表达调控中发挥着重要作用，与肿瘤的 发生发展有着密切的联系。检测肿瘤相关基因的甲基化状态对开发肿瘤诊断、治疗等相关技术及研究肿瘤 发生机制具有重要意义。现对肿瘤细胞DNA 甲基化检测技术的优缺点及应用进行综述，以期为研究者在 选择检测方法时提供参考。 关键词：DNA 甲基化；肿瘤；基因表达；检测技术 中图分类号： R394 ；R730.4　　文献标志码：A New progress on detection and research methods of DNA methylation in tumor cells LAI Yi-Jie, KANG Ya-Ni* (School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China) Abstract: DNA methylation is a common epigenetic modification, which plays an important role in the regulation of gene expression and is closely related to the process of tumorigenesis. Investigating into the methylation patterns in tumor related genes shares tremendous significance in developing technologies for tumor diagnosis and tumor treatment and in understanding the mechanisms underlying tumorigenesis. This review gives an intensive look into the existing DNA methylation detection methods and a brief comment about their relative merits and applications to provide reference for researchers when selecting a proper detection method. Key words: DNA methylation; cancer; gene expression; methylation dete